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对甘肃中西部及宁夏南部不同生态条件下栽培和野生豆科植物根瘤菌资源进行了调查,从36属98种豆科植物根瘤样品中分离得360株根瘤菌,其中44株是从30种未报道过结瘤情况的豆科植物中分离获得。以20属22种寄主植物对大部分菌株进行了回接结瘤实验,回接结瘤率81.2%。它们与豆科寄主植物共生结瘤85%为有效根瘤。不同种根瘤固氮活性差异较大,一般比较低,小于1μmolC2H4?gFWh的占46.6%,大于10μmolC2H4?gFWh的占6.8%,最高者仅37.73μmolC2H4?gFWh。根瘤固氮活性与寄主植物生态及生长发育期有相关性。所有分离菌株均保存在中国科学院新疆生物土壤沙漠研究所微生物研究室及中国农业大学生物学院菌种室。
Rhizobium resources of cultivated and wild legumes under different ecological conditions in central and western Gansu and southern Ningxia were investigated. 360 Rhizobium strains were isolated from 36 legumes and 98 legume nodules samples, of which 44 were from 30 species Reported nodulation of leguminous plants isolated. Back-nodulation experiments were carried out on most of the strains with 22 host plants of 20 genera, with the nodulation rate of 81.2%. They are 85% of commensal nodules with leguminous host plants as effective nodules. The nitrogen fixation activities of different species of nodules are quite different, generally low, 46.6% less than 1μmolC2H4? GFWh, 6.8% more than 10μmolC2H4? GFWh, the highest is only 37.73μmolC2H4? GFWh. Nitrogen fixation activity of nodules is related to host plant ecology and growth and development. All isolates were deposited in the Laboratory of Microbiology, Xinjiang Institute of Biological Deserts, Chinese Academy of Sciences and the Department of Biology, China Agricultural University.