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目的:探讨间歇运动抗心脏缺血再灌注(ischemia/reperfusion,I/R)损伤的保护作用及其机制。方法:32只大鼠随机分成三组:间歇运动训练组、一次间歇运动组和对照组(假手术组和缺血再灌注对照组),缺血再灌注模型制备前,间歇运动训练组进行高强度间歇运动训练,一次间歇运动组仅进行一次高强度的间歇运动,对照组不运动。采用结扎大鼠左冠状动脉方法制备在体大鼠心肌缺血再灌注损伤模型。缺血30分钟、再灌注40分钟后检测大鼠血清心肌肌酸激酶(CK)、乳酸脱氢酶(LDH)活性,心肌抗氧化酶活性和心肌丙二醛(MDA)含量。结果:经间歇运动训练和一次间歇运动预处理的大鼠,血清CK和LDH显著低于对照组,心肌组织SOD和GSH-PX活性显著高于对照组,心肌中MDA含量显著低于对照组。结论:间歇运动训练可能通过提高缺血再灌注心肌的抗氧化能力来实现对其的保护作用。
Objective: To investigate the protective effect and mechanism of intermittent exercise on anti-ischemia / reperfusion (I / R) injury. Methods: Thirty-two rats were randomly divided into three groups: the intermittent exercise training group, the first intermittent exercise group and the control group (sham operation group and ischemia-reperfusion control group), the ischemia-reperfusion model Intensive intermittent exercise training, intermittent exercise group only once a high-intensity intermittent exercise, the control group did not exercise. A rat model of myocardial ischemia-reperfusion injury was established by ligation of left coronary artery in rats. Thirty minutes after ischemia and 40 minutes after reperfusion, creatine kinase (CK), lactate dehydrogenase (LDH) activity, myocardial antioxidant enzyme activity and myocardial MDA content in serum were measured. Results: The serum CK and LDH in rats pretreated with intermittent exercise and intermittent exercise were significantly lower than those in control group. The activities of SOD and GSH-PX in myocardium were significantly higher than those in control group. The content of MDA in myocardium was significantly lower than that in control group. Conclusion: Intermittent exercise training may protect the myocardium by increasing its anti-oxidative capacity.