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在田间条件下,选用3个小麦材料与不同玉米品种进行杂交,用含不同体积分数二甲基亚砜(DMSO)的100 mg/L 2,4-D溶液和3%DMSO与2,4-D配比处理两种方法诱导小麦×玉米产生单倍体.结果表明,在不同DMSO处理中,2%体积分数的DMSO最佳处理平均得胚率最高为15.8%,3%处理最低(7.1%),二者差异显著;在激素配比试验中,以授粉后48 h采用小花滴注100 mg/L 2,4-D溶液、授粉后72 h再次采用小花滴注并穗下茎节注射3%DMSO的100 mg/L 2,4-D溶液的处理得胚率最高(14.1%),但与3%DMSO的2,4-D 1次处理(13.4%)2、,4-D 1次处理(12.7%)、2,4-D 2次处理(10.6%)和3%DMSO的2,4-D 2次处理(12.6%)之间无显著差异,而这5种处理的得胚率均与对照和未授粉只进行3%DMSO的2,4-D 1次处理存在极显著差异.
Under the field conditions, three wheat cultivars were crossed with different maize cultivars, and treated with 100 mg / L 2,4-D solution containing different volume fractions of dimethylsulfoxide (DMSO) and 3% DMSO with 2,4 -D with two kinds of methods to induce haploid of wheat × maize.The results showed that in the different DMSO treatment, the best embryo yield of 2% volume fraction DMSO was 15.8% and the lowest was 3% %), The difference between the two was significant. In the hormonal proportioning experiment, the solution of 100 mg / L 2,4-D was instilled 48 h after pollination and again in 72 h after pollination Treatment with 3% DMSO in a 100 mg / L 2,4-D solution resulted in the highest embryo rate (14.1%) but with 2,4-D in 3% DMSO for 13.4% There was no significant difference between the second treatment (12.7%), 2,4-D 2 treatment (10.6%) and 3% DMSO 2,4-D 2 treatment (12.6%), There was a very significant difference in the rates between the control and non-pollination treatments with 2,4-D 1-times only with 3% DMSO.