Cyclobenzaprine盐酸盐及其片剂是新版美国药典收载品种,测定方法缺少专属性,不能反映产品的稳定性。本文介绍使用高压液相测定其片剂的方法。仪器:具有254nm固定波长检出器的Altex 322型高压液相层析仪;进样采用Water710A型自动进样装置,可定量注样20μl。图谱记录与结果计算采用HP3385A型积分仪。试剂:pH3,0.6%磷酸二氢钾缓冲液;内标采用盐酸萘唑啉的甲醇液mg/ml;标准液中内含cyclobenzaprine盐酸盐与盐酸萘唑啉分别为0.01mg/ml与0.05mg/ml。实验条件:流动相乙腈-0.6%磷酸盐缓冲液(pH3)(75:25);流速1.5ml/min。使用Zorbax C-8硅烷化硅胶柱(25×0.46cm内径),用毕次日,层析柱依次用75%乙腈水溶液和甲醇冲 Cyclobenzaprine hydrochloride and its tablets are new versions of the United States Pharmacopoeia, which lack specificity and do not reflect the stability of the product. This article describes a method for measuring tablets using high pressure liquid phase. Instrument: Altex 322 High Pressure Liquid Chromatograph with 254nm Fixed Wavelength Detector; Injection Using Water710A Autosampler, Quantitative Injection 20μl. Atlas record and result calculation using HP3385A integrator. Reagents: pH 3, 0.6% potassium dihydrogen phosphate buffer; internal standard solution of naphthalene hydrochloride methanol solution mg / ml; standard solution containing cyclobenzaprine hydrochloride and naphtazoline hydrochloride were 0.01mg / ml and 0.05mg / ml. Experimental conditions: mobile phase acetonitrile -0.6% phosphate buffer (pH 3) (75:25); flow rate 1.5 ml / min. A Zorbax C-8 silanized silica gel column (25 × 0.46 cm ID) was used. On the next day, the column was washed with 75% acetonitrile in water and methanol