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背景:参乌胶囊是由首都医科大学宣武医院药理研究室自行研制用于治疗老年痴呆的纯中药制剂,对多种拟痴呆模型动物的学习记忆能力有很好的改善作用。目的:观察拟杭廷顿病(Huntingtondisease,HD)模型大鼠学习记忆能力改变及参乌胶囊对其的干预作用,探讨其可能的作用机制。设计:随机对照的实验研究。地点和材料:实验地点:首都医科大学宣武医院药物研究中心。雄性SD大鼠60只,随机分为正常对照组,3-硝基丙酸(3-nitropropionicacid,3-NPA)模型组,参乌胶囊小剂量、中剂量和大剂量组,氟哌啶醇组。每组10只。在实验进行中,正常对照组死亡1只,3-NPA模型组和参乌胶囊小剂量组各死亡2只,氟哌啶醇组死亡3只,考虑死亡原因为吸入性窒息。干预:3-NPA模型组用3-NPA20mg/kg对SD大鼠隔日腹腔注射1次,共20d,建立拟HD大鼠模型。造模的同时,参乌胶囊小剂量(0.45g/kg)组,中剂量(0.9g/kg)和大剂量(1.8g/kg)组大鼠给予参乌胶囊连续灌胃25d。氟哌啶醇组自造模当日起给予氟哌啶醇,起始剂量为100μg/(kg·d),隔日递增,直至1000μg/(kg·d)。主要观察指标:大鼠在Morris水迷宫中的游出时间和距离;在避暗实验中的潜伏期和错误次数;海马处胆碱乙酰基转移酶活性;大鼠海马CA4区胶质细胞源性神经生长因子的细胞数量及细胞面积。结果:Morris?
BACKGROUND: Shenwu Capsule is a pure Chinese medicine preparation developed by the Xuanwu Hospital Pharmacology Research Institute of Capital Medical University for the treatment of Alzheimer’s disease. It has a good effect on improving the learning and memory ability of many animal models of dementia. OBJECTIVE: To observe the changes of learning and memory abilities of the model rats with Huntington disease (HD) and the intervention effects of Shenwu capsule on it, and to explore its possible mechanism. Design: Randomized controlled experimental study. Location and Materials: Experimental site: Drug Research Center, Xuanwu Hospital, Capital Medical University. 60 male SD rats were randomly divided into normal control group, 3-nitropropionic acid (3-NPA) model group, Shenwu capsule low-, medium- and high-dose group, haloperidol group. . 10 in each group. During the experiment, one death occurred in the normal control group, two deaths occurred in the 3-NPA model group and the Shenwu capsule low dose group, and three died in the haloperidol group. The cause of death was considered as inhalation asphyxia. Intervention: 3-NPA model group was injected intraperitoneally with 3-NPA 20mg/kg once every other day for 20 days to establish a model of HD rat. Simultaneously with the modeling, Shenwu capsules were given to Shenwu capsules in small dose (0.45g/kg) group, medium dose (0.9g/kg) and high dose (1.8g/kg) groups for 25 days. The haloperidol group was given haloperidol starting from the date of the model, and the starting dose was 100 μg/(kg·d), which was increased every other day up to 1000 μg/(kg·d). MAIN OUTCOME MEASURES: Time and distance traveled by rats in the Morris water maze; Latency and number of errors in dark-sweeping experiments; Choline acetyltransferase activity in the hippocampus; Glial cell-derived neurons in the hippocampal CA4 region of rats The number of cells and cell area of growth factors. Result: Morris?