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目的观察αvβ3 mRNA和αvβ3蛋白在胃癌组织和正常组织中的表达及其与胃癌临床病理参数的关系。方法应用原位杂交和免疫组织化学染色SP法检测80例胃癌组织和50例正常胃黏膜组织中αvβ3 mRNA及其蛋白的表达。结果胃癌组织中αvβ3 mRNA及其蛋白的阳性表达率分别是75.0%和67.5%,明显高于癌旁组织,差异有统计学意义(P<0.05)。αvβ3 mRNA和其蛋白的表达与分化程度、病理分期和淋巴结转移有显著相关性(P<0.05),与年龄和性别无相关性(P>0.05),αvβ3 mRNA与其蛋白在胃癌中的表达呈正相关性(r=0.339,P<0.05)。结论αvβ3基因在胃癌的发生、发展中起着重要作用,在胃癌中高表达可能是胃癌恶化的一个标志。
Objective To observe the expression of αvβ3 mRNA and αvβ3 protein in gastric carcinoma and normal tissues and their relationship with clinicopathological parameters. Methods The expression of αvβ3 mRNA and protein in 80 cases of gastric carcinoma and 50 cases of normal gastric mucosa were detected by in situ hybridization and immunohistochemistry. Results The positive rates of αvβ3 mRNA and protein in gastric cancer tissues were 75.0% and 67.5%, respectively, which were significantly higher than those in paracancerous tissues (P <0.05). There was a significant correlation between the expression of αvβ3 mRNA and its differentiation, pathological stage and lymph node metastasis (P <0.05), and age and sex (P> 0.05). The expression of αvβ3 mRNA was positively correlated with its protein expression in gastric cancer (R = 0.339, P <0.05). Conclusion αvβ3 gene plays an important role in the occurrence and development of gastric cancer. High expression in gastric cancer may be a sign of gastric cancer.