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[目的]观察不同保存条件对血清CEA、CA199、AFP浓度的影响。[方法]取结直肠癌患者血清样品,在取血当时、冷藏(4±1)℃保存3周,以及室温(28±3)℃保存的条件下,采用酶免疫分析法(EIA),测定血清中CEA、CA199、AFP的浓度并进行比较,考察其稳定性。[结果]冰箱冷藏保存1、2、3周及室温保存3 d的血清测定结果均与取血当时测定结果无统计学差异(P>0.05)。室温保存1、2周的血清测定结果明显高于取血当时测定结果(P<0.05)。[结论]CEA、CA199、AFP血清样品在冷藏条件下可稳定保存3周,室温下可稳定保存3 d。
[Objective] To observe the effect of different storage conditions on serum CEA, CA199, AFP concentration. [Methods] Serum samples from patients with colorectal cancer were collected and assayed by enzyme-linked immunosorbent assay (EIA) at the time of blood collection, preservation at 4 ± 1 ℃ for 3 weeks and at room temperature (28 ± 3) ℃, Serum CEA, CA199, AFP concentrations and compared to investigate the stability. [Result] The results of the determination of serum in refrigerators for 1, 2, and 3 weeks and 3 days at room temperature had no statistical difference (P> 0.05). Serum samples at 1 and 2 weeks of storage at room temperature were significantly higher than those at the time of sampling (P <0.05). [Conclusion] The serum samples of CEA, CA199 and AFP can be stably stored for 3 weeks under refrigerated conditions and can be stably stored for 3 days at room temperature.