论文部分内容阅读
目的 探讨β -环连蛋白在肾癌发生发展过程中的作用及其变化机制。方法 我们采用免疫组织化学、Westernblot、RT -PCR等方法对本院收治的 2 6例肾癌患者手术标本进行了研究。结果 2 6例患者中有 2 5例癌组织中细胞胞浆内β 环连蛋白表达明显高于正常组织内 ,而且肿瘤分期越高 ,癌细胞内β 环连蛋白的表达也越强 ,pT3和pT4期的肿瘤内的表达明显高于pT1和pT2期的肿瘤 ,P <0 .0 1,而其β -环连蛋白mRNA的表达水平并无明显变化。结论 细胞内 β -环连蛋白表达的增加与肾癌的发生发展有关 ,而且可能是肾癌形成和发展的重要机制之一。
Objective To investigate the role and mechanism of β-catenin in the development of renal cell carcinoma. Methods We used immunohistochemistry, Western blot, RT-PCR and other methods to study the surgical specimens of 26 patients with renal cell carcinoma treated in our hospital. RESULTS: The expression of β-catenin in cytoplasm was significantly higher in 25 of 26 patients than in normal tissues. The higher the tumor stage, the stronger the expression of β-catenin in cancer cells, pT3 and The expression in pT4 tumors was significantly higher than that in pT1 and pT2 tumors, P < 0.01, while the expression level of β-catenin mRNA was not significantly changed. Conclusion The increase of intracellular β-catenin expression is related to the occurrence and development of renal cell carcinoma, and may be one of the important mechanisms of the formation and development of renal carcinoma.