论文部分内容阅读
目的建立以高效液相色谱法同时测定乳膏中尿囊素和烟酰胺含量的方法。方法采用Zorbax SB C18色谱柱(4.6 mm×250 mm,5μm),流动相:甲醇-0.02 mol.L 1磷酸二氢钾溶液(25∶75),流速:0.5 mL.min 1,柱温:30℃,检测波长:217 nm。结果尿囊素在4.2～21.0μg.L 1内线性关系良好(r=0.999 9),烟酰胺在8.0～40.0μg.L 1内线性关系良好(r=0.999 7);尿囊素和烟酰胺平均加样回收率分别为99.4%(RSD=0.27%)和99.7%(RSD=0.25%)。结论该方法简便、快速、准确,可用于制剂的质量控制。
Objective To establish a method for the simultaneous determination of allantoin and niacinamide in cream by high performance liquid chromatography. Methods A Zorbax SB C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of methanol-0.02 mol·L -1 potassium dihydrogen phosphate solution (25:75), the flow rate was 0.5 mL.min 1 and the column temperature was 30 ℃, detection wavelength: 217 nm. Results The linearity of allantoin in the range of 4.2 ~ 21.0μg.L 1 was good (r = 0.999 9), nicotinamide was good in the range of 8.0 ~ 40.0μg.L 1 (r = 0.999 7), allantoin and nicotinamide The average recovery rates were 99.4% (RSD = 0.27%) and 99.7% (RSD = 0.25%), respectively. Conclusion The method is simple, rapid and accurate and can be used for the quality control of the preparation.