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为了揭示人工甘蓝型油菜早期世代遗传和表观遗传变异规律,以A组合(大黄油菜×中花芥蓝)S0世代、B组合(大黄油菜×中迟芥蓝)S0和S1世代人工甘蓝型油菜为材料,分别利用AFLP和MSAP技术检测基因组变化及甲基化模式变化情况。结果表明,16对引物在A组合S0扩增到523条带,其中4对引物扩增出9条变异带,包括7条亲本缺失带和2条新增带,分别占S0总条带的1.33%和0.38%;45对引物在B组合双亲植株扩增到1093条带,只有1对引物检测到1条父本带型在所有S0植株中缺失,约占S0总条带的0.09%;在B9子代F19-1～F19-16总共扩增得到1092条带,变异带有10条,占总条带的0.915%,其中包括9条缺失带和1条新增带,9条缺失带全部位于C基因组。MSAP检测发现,B组合S0植株中有3个位点发生了甲基化模式的改变,全部位于A基因组,甲基化模式改变位点占总检测位点的1.37%。研究还发现B组合S0世代一个植株出现可遗传的花色变异,推测该表型变异与B组合人工甘蓝型油菜中C基因组变异有关。
In order to reveal the genetic and epigenetic variation of early generations of artificial Brassica napus, A (Rutaceae × Scutellaria barbata) S0 generation, B combination (Rhubarb × Zingiber officinale) S0 and S1 generations of Brassica napus As materials, the changes of genomic and methylation patterns were detected by AFLP and MSAP respectively. The results showed that 16 pairs of primers amplified 523 bands in the A combination S0, of which 9 pairs of primers amplified 9 variation bands, including 7 parental deletion bands and 2 newly added bands, accounting for 1.33 % And 0.38%, respectively. Among 45 pairs of primers, 1093 bands were amplified from B parents, and only one pair of primers detected one deletion in all S0 plants, accounting for 0.09% of the total S0 bands. A total of 1092 bands were amplified from F19-1 to F19-16 in progeny of B9, with 10 variations, accounting for 0.915% of total bands, including 9 deletion bands and 1 new addition band, and 9 deletion bands Located in the C genome. The results of MSAP showed that the methylation patterns of all three S loci in group B were all changed in A genome, and the methylation patterns changed in 1.37% of the total loci. The study also found that the B combination of S0 generation of a plant appears heritable flower color variation, suggesting that the phenotypic variation and B combination of artificial Brassica napus genome C mutation.