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目的探讨浒苔多糖对四氧嘧啶致1型糖尿病小鼠抗糖尿病作用的分子机制。方法采用四氧嘧啶造糖尿病模型,给予低、中、高剂量(200,400,800 mg/kg)浒苔多糖灌胃,连续28 d。使用反转录-聚合酶链式反应测定相关基因表达情况。结果模型组、浒苔多糖中、高剂量组和二甲双胍组Bax基因表达水平分别为(0.86±0.16)、(0.52±0.14)、(0.46±0.11)、(0.59±0.26),差异具有统计学意义(P<0.05或P<0.01);模型组、高剂量浒苔多糖组Bcl-2基因表达水平分别为(0.17±0.13)、(0.34±0.14),差异具有统计学意义(P<0.05);模型组、浒苔多糖低、中、高剂量组和二甲双胍组MnSOD基因表达水平分别为(0.88±0.24)(2.63±0.97)、(2.73±0.87)、(3.23±0.57)、(1.74±0.73),差异具有统计学意义(P<0.05或P<0.01)。结论浒苔多糖能纠正糖尿病小鼠糖、脂代谢紊乱,其机制可能与其抗氧化应激与抗凋亡作用有关。
Objective To investigate the molecular mechanism of Enteromorpha polysaccharides on the antidiabetic effect of alloxan-induced type 1 diabetic mice. Methods Alloxan-induced diabetic model was established. Low, medium and high doses of Enteromorpha polysaccharides (200,400,800 mg / kg) were given orally for 28 consecutive days. Reverse transcriptase-polymerase chain reaction was used to determine gene expression. Results The expression levels of Bax gene in model group, Enteromorpha polysaccharide, high dose group and metformin group were (0.86 ± 0.16), (0.52 ± 0.14), (0.46 ± 0.11) and (0.59 ± 0.26) respectively, with statistical significance (P <0.05 or P <0.01). The expression of Bcl-2 gene in model group and high dose Enteromorpha polysaccharides group were (0.17 ± 0.13) and (0.34 ± 0.14) respectively, with statistical significance (P <0.05) The MnSOD gene expression levels of model group, Enteromorpha polysaccharides in low, medium and high dose groups and metformin group were (0.88 ± 0.24) (2.63 ± 0.97), (2.73 ± 0.87), (3.23 ± 0.57) and (1.74 ± 0.73) , The difference was statistically significant (P <0.05 or P <0.01). Conclusions Polysaccharide of Enteromorpha prolifera can correct the disorder of glucose and lipid metabolism in diabetic mice. The mechanism may be related to its anti-oxidative stress and anti-apoptotic effects.