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目的了解四川省食品从业人员中金黄色葡萄球菌携带情况及其耐药现状,为食品安全风险评估提供参考。方法选择绵阳市、德阳市、宜宾市等6市县的大型食堂作为采样点,采集相关食品从业人员双手的涂抹样。对分离的可疑致病菌进行鉴定、药敏试验、PCR耐药基因检测和金黄色葡萄球菌A蛋白(SPA)分型。结果采集1 072份样本,阳性样本124份,检出率为11.57%;其中13株为MRSA,苯唑西林耐药率为10.48%,多重耐药占31.45%。13株MRSA中,11株携带mec A基因。SPA分型有5个型别。结论四川省食品从业人员中金黄色葡萄球菌携带率较高,耐药情况严重,且菌株型别分布具有地理差异。有必要采取适当措施避免群体性食物中毒事件的发生。
Objective To understand the situation of drug-resistant Staphylococcus aureus in food workers in Sichuan and to provide a reference for food safety risk assessment. Methods Mianyang City, Deyang City, Yibin City, six counties and other large canteens as a sampling point, gathers samples of food-related personnel hands. Identification of isolated suspicious pathogens, drug susceptibility testing, PCR resistance gene detection and Staphylococcus aureus protein (SPA) typing. Results A total of 1 072 samples were collected and 124 positive samples were collected. The detection rate was 11.57%. 13 strains were MRSA, the oxacillin resistance rate was 10.48%, and multi-drug resistance was 31.45%. Among 13 strains of MRSA, 11 strains carried mec A gene. There are 5 types of SPA typing. Conclusion The prevalence of Staphylococcus aureus in food workers in Sichuan Province is high, the drug resistance is serious, and the distribution of strain type has geographical difference. It is necessary to take appropriate measures to prevent the occurrence of mass food poisoning.