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以大豆东农42和东农47为试验材料,采用盆栽方式,利用茚三酮显色,甲苯萃取分光光度法测定,研究在苗期、开花期分别喷施不同浓度稀土元素La(Ⅲ)和Ce(Ⅲ)对大豆叶片脯氨酸含量的影响。结果表明:La(Ⅲ)和Ce(Ⅲ)在一定浓度下均能促进大豆叶片脯氨酸的合成,且La(Ⅲ)的促进作用好于Ce(Ⅲ),开花期处理好于苗期;对于大豆东农42和东农47,开花期的120 mg·L-1La Cl3处理,脯氨酸含量均达到最大值分别为13.33和13.84μg·g-1,二者增幅分别为10.20%和13.63%,与对照之间均呈显著性差异。
Using soybean Dongnong 42 and Dongnong 47 as experimental materials, pot experiments were carried out. The ninhydrin color and toluene extraction spectrophotometry were used to study the effects of different concentrations of rare earth elements La (Ⅲ) and Effect of Ce (Ⅲ) on Proline Content in Soybean Leaves. The results showed that La (Ⅲ) and Ce (Ⅲ) promoted the proline synthesis of soybean leaves at a certain concentration, and the promoting effect of La (Ⅲ) was better than that of Ce (Ⅲ) and the flowering stage was better than the seedling stage. For soybean Dongnong 42 and Dongnong 47, the maximum proline content reached 13.33 and 13.84 μg · g-1 at the flowering stage of 120 mg · L-1La Cl3, respectively, with the increase rate of 10.20% and 13.63 %, With the control showed significant differences.