本研究克隆了1个陆地棉新的钙依赖蛋白激酶基因GhCPK5(Genbank登录号为:HM002634)。GhCPK5基因组DNA序列全长3 031 bp,包含一个1 707 bp的开放阅读框,具有7个外显子和6个内含子。GhCPK5可能定位于细胞核内,有568个氨基酸残基,分子量为63 ku。氨基酸序列分析表明,陆地棉GhCPK5与杨树PtCPK5同源性达到89%,同样包含完整的激酶结构域和4个EF-hand结构域。RT-PCR分析表明,该基因在根、茎、叶等组织中表达;在盐胁迫处理下,GhCPK5在各组织的表达量明显上升。 This study cloned a new cotton GhCPK5 (GenBank accession number: HM002634). The GhCPK5 genomic DNA sequence is 3 031 bp in length and contains a 1 707 bp open reading frame with 7 exons and 6 introns. GhCPK5 may be located in the nucleus, with 568 amino acid residues, molecular weight of 63 ku. Amino acid sequence analysis showed that GhCPK5 had 89% homology with poplar PtCPK5, which also contained intact kinase domain and four EF-hand domains. RT-PCR analysis showed that the gene was expressed in the tissues of roots, stems and leaves. Under salt stress, the expression of GhCPK5 in various tissues was significantly increased.