建立同时分析草酸、酒石酸、苹果酸和柠檬酸的RP-HPLC法。色谱柱为Agilent Eclipse XDB-C18(4.6mm×250 mm,5μm),流动相为0.01 mol/L KH_2PO_4水溶液-甲醇(95∶5,V/V),pH 2.5,流速为1.0 mL/min,检测波长为210 nm,柱温为室温,进样量20μL。在建立的色谱条件下,各酸性组分获得良好的分离,质量浓度与峰面积之间呈良好线性关系,相关系数r>0.999,草酸、酒石酸和苹果酸的检出限分别为0.000 3,0.000 4和0.000 6 mg/mL。把所建立的RP-HPLC法用于酢浆草不同部位乙醇提取物酸性组分的分析,结果表明:样品液中未检出柠檬酸,在茎叶中各酸性组分含量为苹果酸>草酸>酒石酸,而在根部中为苹果酸>酒石酸>草酸,酢浆草不同部位提取物酸性组分含量存在一定的差异,其中草酸含量的差异最显著。在0.010 6~0.492 mg/mL加标浓度范围内样品各酸性组分加标回收率在99.0%~105.9%之间,RSD≤1.7%。所建立的RP-HPLC法操作简便,精密度高,可靠,适用于酢浆草中酸性成分含量的测定。 RP-HPLC method for the simultaneous analysis of oxalic acid, tartaric acid, malic acid and citric acid was established. The column was Agilent Eclipse XDB-C18 (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of 0.01 mol / L aqueous KH_2PO_4 solution (95: 5 V / V) Wavelength of 210 nm, the column temperature is room temperature, injection volume 20μL. Under the established chromatographic conditions, the acidic components were well separated, and the linear relationship was obtained between the mass concentration and the peak area, the correlation coefficient r> 0.999, the detection limits of oxalic acid, tartaric acid and malic acid were 0.000 3, 0.000 4 and 0.000 6 mg / mL. The established RP-HPLC method was used to analyze the acidic components of ethanol extract from different parts of Sorrel. The results showed that citric acid was not detected in the sample solution, and the contents of acid components in the stem and leaf were malic acid> oxalic acid > Tartaric acid, while there were some differences in the content of acidic components in different parts of malic acid> tartaric acid> oxalic acid and oxalic acid in roots, of which the difference was the most significant. The spiked recoveries of various acidic components in the range of 0.010 6 ~ 0.492 mg / mL ranged from 99.0% to 105.9% with the RSD≤1.7%. The established RP-HPLC method is simple, precise and reliable, and is suitable for the determination of the acid component content in the oxalis.