论文部分内容阅读
以甲基磺酸乙酯（ＥＭＳ）为致突变的阳性对照物，观察了人胎盘提取物（ＨＰＥ）对ＥＭＳ致果蝇伴性隐性致死突变的抑制作用。结果表明，ＥＭＳ的致死突变率为９．０７１％；ＥＭＳ染毒后喂饲含１０％、５％和２．５％ＨＰＥ培养基的３组果蝇，其致死突变率分别为６．１８４％、６．７２０％和７．９０７％，与ＥＭＳ阳性组比较，大、中二剂量组明显降低，Ｐ＜０．０５。喂饲ＨＰＥ果蝇的精子、精细胞和精母细胞阶段的伴性隐性致死突变率分别为８．２３５％、６．６４９％和５．９０６％，均低于ＥＭＳ阳性组中各细胞阶段的致死突变率，Ｐ＜０．０５。表明ＨＰＥ可抑制ＥＭＳ的致基因突变作用，且对不同阶段的生殖细胞有一定的保护作用。
The inhibitory effect of human placenta extract (HPE) on the sexual recessive mutations in Drosophila melanogaster was observed by using methyl methanesulfonate (EMS) as the mutagenic positive control. The results showed that the lethal mutation rate of EMS was 9.071%. The lethal mutation rates of three groups of Drosophila fed with 10%, 5% and 2.5% HPE medium after EMS exposure were 6.184% , 6.720% and 7.907%, respectively. Compared with the EMS-positive group, the levels of big and middle two-dose group decreased obviously, P <0.05. The rates of the associated recessive lethal mutations in sperm, sperm cells and spermatocytes of HPE-fed flies were 8.235%, 6.649% and 5.906%, respectively, which were all lower than those in EMS-positive group The lethal mutation rate, P <0.05. This indicates that HPE can inhibit the mutagenesis induced by EMS and protect the germ cells in different stages.