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目的建立胰岛素抵抗细胞模型,探讨岩藻黄素体外改善胰岛素抵抗及降血糖活性。方法采用高浓度胰岛素诱导HepG2细胞,筛选最佳诱导条件建立IR-HepG2细胞模型。用不同浓度岩藻黄素处理模型细胞,用葡萄糖氧化酶法(GOD-POD)检测岩藻黄素对IR-HepG2细胞葡萄糖消耗量的影响。结果 10-6 mol/L胰岛素处理细胞24h为建立IR-HepG2细胞模型的最佳条件。5~20μmol/L的岩藻黄素均可促进IRHepG2细胞对葡萄糖的利用,10、12.5和15μmol/L组的糖消耗量显著高于模型组(P<0.01)。结论岩藻黄素可改善体外IR-HepG2细胞的胰岛素抵抗,促进IR-HepG2细胞的葡萄糖消耗,具有改善胰岛素抵抗和降血糖活性。
Objective To establish a model of insulin resistance cells and explore the effect of fucoxanthin on improving insulin resistance and hypoglycemic activity in vitro. Methods HepG2 cells were induced with high concentration of insulin and the best induction conditions were screened to establish IR-HepG2 cell model. The model cells were treated with different concentrations of fucoxanthin, and glucose oxidase (GOD-POD) was used to detect the effect of fucoxanthin on glucose consumption in IR-HepG2 cells. Results The optimal conditions for establishing IR-HepG2 cell model were treated with 10-6 mol / L insulin for 24 h. 5 ~ 20μmol / L fucoxanthin can promote glucose utilization in IRHepG2 cells, and the sugar consumption in 10, 12.5 and 15μmol / L groups was significantly higher than that in model group (P <0.01). Conclusion Fucoxanthin can improve the insulin resistance of IR-HepG2 cells in vitro and promote the glucose consumption of IR-HepG2 cells, which can improve the insulin resistance and hypoglycemic activity.