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目的 探讨肺癌形成发展过程中血清鳞癌相关抗原 (SCCAg)、癌胚抗原 (CEA)及糖链抗原 15 3(CA15 3 )的动态变化 ,为寻找有意义的肺癌早期诊断指标提供依据。方法 经肺叶支气管内灌注甲基胆蒽(MCA)碘油溶液对 91只Wistar大鼠诱癌 ,分别于灌注后第 2 0、40、5 0、60、70、80天分批处死动物并采取血清。从经病理确诊为不典型增生、原位癌及早期浸润癌各阶段的动物模型组中各选取 3～ 7例 ,应用微粒子酶免疫试验技术 (MEIA)测定血清中SCCAg、CEA、CA15 3的水平。结果 在不典型增生、原位癌阶段 ,三种标志物均无改变 ;但在早期浸润癌阶段 ,血清SCCAg明显增高 ,其差异有统计学意义 (P <0 .0 1) ,而CEA、CA15 3无明显改变。结论 血清SCCAg在肺鳞癌早期即升高 ,表明SCCAg是肺鳞癌早期诊断的一个很有意义的指标。
Objective To investigate the dynamic changes of SCCAg, CEA and CA15 3 in the development and progression of lung cancer and provide the basis for searching for the early diagnosis of lung cancer. Methods Ninety Wistar rats were induced by intratracheal intratracheal instillation of methylcholanthrene (MCA) iodine solution. Animals were sacrificed on days 20, 40, 50, 60, 70, serum. Three to seven cases were selected from animal models which were pathologically diagnosed as atypical hyperplasia, carcinoma in situ and early invasive carcinoma. Serum levels of SCCAg, CEA and CA15 3 were measured by microparticle enzyme immunoassay (MEIA) . Results In atypical hyperplasia and carcinoma in situ, there was no change in all three markers. However, in the early stage of invasive carcinoma, serum SCCAg was significantly higher (P <0.01), but CEA, CA15 3 no significant change. Conclusion Serum SCCAg increased in the early stage of squamous cell carcinoma of the lung, indicating that SCCAg is a very meaningful indicator for the early diagnosis of squamous cell lung cancer.