OBJECTIVES: To study the structure specificity of Echinococcus granulosus 95 (Eg95) gene and theopen reading frame (ORF) of the full-length cDNA sequence in Xinjiang, northwestern China andconstruct Eg95 Xinjiang strain DNA vaccine.METHODS: Primers of Eg95 were designed on the basis of the sequence of Eg95 antigen cDNA.Genomic DNA was extracted from E. granulosus protoscoleces (sheep) in Xinjiang. The Eg95 gene andfull-length Eg95 cDNA were amplified by PCR from the genomic DNA and protoscolex cDNA library ofE. granulosus in Xinjiang, respectively. The Eg95 gene was cloned into pUCm-T plasmid and the Eg95cDNA into eukaryotic expression plasmid pcDNA3 for the construction of full-length ORF DNA vaccinepcDNA3-Eg95/XJ. Both Eg95 gene and Eg95 cDNA were sequenced and analyzed by DNAman andNCBI/Blast program.RESULTS: DNA sequence analysis of Eg95 Xinjiang strain (Eg95/XJ) cDNA fragment indicated thatthe coding region of the full-length of Eg95/XJ was 471bp and that encoding a peptide with 156aa and thegenomic DNA size was 1191bp. Homological comparison showed that the ORF of Eg95/XJ cDNA wasidentical to the cDNA sequence of Eg95 reported in the reading frame, but the genomic DNA was anew sequence, named Eg95/XJ and the multiple nucleotide differences, which were represented inEg95/XJ gene in comparison with those of the New Zealand strain, occurred predominantly in thenon-coding regions of the gene. The pcDNA3-Eg95/XJ positive clone was the exact recombinant plasmidand could be used ms a DNA vaccine.CONCLUSION: pcDNA3-Eg95/XJ Xinjiang strain DNA vaccine is successfully constructed. OBJECTIVES: To study the structure specificity of Echinococcus granulosus 95 (Eg95) gene and the reading frame (ORF) of the full-length cDNA sequence in Xinjiang, northwestern China and construct Eg95 Xinjiang strain DNA vaccine. METHODS: Primers of Eg95 were designed on the The Eg95 gene was amplified by PCR from the genomic DNA and protoscolex cDNA library of E. granulosus in Xinjiang, respectively. The Eg95 gene was cloned into pUCm-T plasmid and the Eg95 cDNA into eukaryotic expression plasmid pcDNA3 for the construction of full-length ORF DNA vaccine pcDNA3-Eg95 / XJ. Both Eg95 gene and Eg95 cDNA were sequenced and analyzed by DNAman and NCBI / Blast program. RESULTS: DNA sequence analysis of Eg95 Xinjiang strain (Eg95 / XJ) cDNA fragment indicated that the coding region of the full-length of Eg95 / XJ was 471 bp and that encoding a peptide with 1 56aa and the genomic DNA size was 1191bp. Homological comparison showed that the ORF of Eg95 / XJ cDNA wasidentical to the cDNA sequence of Eg95 reported in the reading frame, but the genomic DNA was anew sequence, named Eg95 / XJ and the multiple nucleotide differences, which were represented in Egg95 / XJ gene in comparison with those of New Zealand strain, predominantly in thenon-coding regions of the gene. The pcDNA3-Eg95 / XJ positive clone was the exact recombinant plasmid and could be used ms a DNA vaccine. CONCLUSION : pcDNA3-Eg95 / XJ Xinjiang strain DNA vaccine is successfully constructed.