应用显微切割TRAP法对食管癌端粒酶活性进行分析

来源 :中华医学杂志 | 被引量 : 0次 | 上传用户:zq19900303
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的 探讨端粒酶活性在食管粘膜上皮不典型增生、食管鳞癌中的变化 ,同时研究端粒酶活性与癌的分化、浸润、转移的关系。方法 应用显微切割 TRAP 银染法对 4 5例食管鳞癌组织、癌旁非典型增生上皮及切缘的正常粘膜上皮进行定点端粒酶活性的检测。结果 食管正常粘膜上皮的端粒酶活性阳性率为 5 % (2 / 4 0 ) ,非典型增生上皮为 79 3% (2 3/ 2 9) ,癌组织为 82 2 % (37/ 4 5 )。非典型增生上皮的端粒酶活性明显高于正常上皮 ,差异有显著意义 (P <0 0 1)。同一癌组织中不同分化程度、不同浸润深度癌巢的端粒酶活性差异无显著意义 (P >0 0 5 )。有淋巴结转移者癌组织端粒酶活性明显高于无淋巴结转移者 ,差异有显著意义 (P <0 0 5 )。结论 食管鳞癌及癌旁不典型增生组织端粒酶活性明显增高。癌组织中端粒酶活性与分化程度无关 ,而与淋巴结转移有关 Objective To investigate the changes of telomerase activity in esophageal epithelial dysplasia and esophageal squamous cell carcinoma and to study the relationship between telomerase activity and differentiation, invasion and metastasis of esophageal mucosa. Methods Microdissection TRAP silver staining was used to detect the telomerase activity in 45 cases of esophageal squamous cell carcinoma, adjacent atypical hyperplastic epithelium and normal mucosal epithelium of the margin. Results The positive rate of telomerase activity in normal esophageal epithelium was 5% (2/40), that of atypical hyperplastic epithelium was 79 3% (2 3/29) and that of cancerous tissue was 82 2% (37/45) . The telomerase activity of atypical hyperplastic epithelium was significantly higher than that of normal epithelium (P <0.01). There was no significant difference in telomerase activity in cancer nests with different degree of differentiation and depth of invasion in the same cancer (P> 0.05). The telomerase activity in cancerous tissues with lymph node metastasis was significantly higher than those without lymph node metastasis, the difference was significant (P <0 05). Conclusion The telomerase activity in esophageal squamous cell carcinoma and paraneoplastic atypical hyperplasia was significantly higher. Telomerase activity in cancer tissue was not related to the degree of differentiation, but was related to lymph node metastasis
其他文献
目的:探讨扩髓交锁髓内钉治疗胫骨不稳定性骨折的治疗效果.方法:对42例胫骨不稳定性骨折病人行扩髓交锁髓内钉治疗,闭合性骨折22例,开放性骨折20例,全部应用静力性固定.结果:
目的研究P27KIP1、P53、P21WAF1/CIP1蛋白表达与胃癌临床病理及预后的关系。方法应用免疫组织化学SP法检测90例胃癌组织中P27KIP1、P53、P21WAF1/CIP1的表达。结果90例胃癌组
通过对近年来的19篇文献进行分析发现外伤性视神经经损伤主要发生在眉额部受到外力打击后,不一定伴有视神经管骨折,以伤后立即失明和伴有骨折者损伤较重,且疗效不理想.及时的
目的探讨成人同种异体肝移植术的麻醉处理及术中生理及血清电解质的变化.方法所有患者选用静脉复合麻醉,术中监测动脉压、中心静脉压、心电图、血液气体、电解质、体温等. 结
目的 研究Graves病 (GD)甲状腺细胞凋亡和凋亡相关蛋白Fas、可溶性Fas(sFas)及Fas配基 (Fas L)的表达特征 ,探讨Fas介导的细胞凋亡与GD发病机制之间的内在联系。方法甲状腺
多年来,传统的三环类抗抑郁剂作为治疗抑郁症的主要药物,取得了良好的临床疗效和社会效益,但因其见效慢,副作用明显,药物依从性较差,不仅延长了患者的疾苦,还增加了引起严重
Objective To establish an experimental model of symptomatic cerebral vasospasm(CVS) after subarachnoid hemorrhage( SAH) in rabbits. Method 2 weeks after the lig
目的:探讨SC-58125 对HepG-2细胞增殖和凋亡的作用及其分子机理.方法:应用细胞培养、MTT、TUNEL、流式细胞光度术、琼脂糖凝胶电泳及Western blot等方法研究SC-58125 对HepG-
目的探索背阔肌皮瓣游离移植对感染创面修复的疗效观察.方法临床治疗12例小腿及足跟部大面积软组织缺损骨外露、骨髓炎形成伴有伤面感染者,切取背阔肌皮瓣面积为10 cm×16 cm
目的 通过检测系统性红斑狼疮 (SLE)患者外周血单个核细胞 (PBMC)分泌细胞因子白细胞介素 10 (IL 10 )、干扰素 γ (IFN γ)水平 ,探讨T淋巴细胞亚群与SLE发病关系。方法