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目的观察大剂量睾酮对雌性小鼠肾脏酶细胞化学的影响。②方法应用电镜细胞化学方法，观察了大剂量睾酮对雌性小鼠肾小管上皮细胞线粒体标志酶（细胞色素Ｃ氧化酶）和质膜标志酶（Ｎａ＋，Ｋ＋－ＡＴＰ酶）活性的影响。③结果对照组小鼠近端和远端小管上皮细胞内细胞色素Ｃ氧化酶活性呈高电子密度，睾酮组小鼠近端及远端小管上皮细胞内线粒体的细胞色素Ｃ氧化酶活性明显减弱，电子密度降低，其远端和近端小管上皮细胞的质膜内褶处Ｎａ＋，Ｋ＋－ＡＴＰ酶活性明显减弱。④结论大剂量睾酮可引起肾小管上皮细胞超微结构的改变，以及与能量代谢等功能有关的酶活性减弱
Objective To observe the effects of high-dose testosterone on the enzymatic cytochemistry in the kidney of female mice. Methods The effects of high-dose testosterone on the activity of mitochondrial enzyme (cytochrome C oxidase) and plasma membrane-bound enzyme (Na +, K + -ATPase) in renal tubular epithelial cells of female mice were observed by electron microscopy. ③ Results The cytochrome C oxidase activity in proximal and distal tubule epithelial cells of mice in control group showed high electron density. The activity of mitochondrial cytochrome C oxidase in proximal and distal tubule epithelial cells of testosterone group was significantly decreased, The electron density decreased and the Na +, K + -ATPase activity in the plasma membrane folds of distal and proximal tubule epithelial cells decreased significantly. ④ Conclusion High-dose testosterone can cause changes in the ultrastructure of renal tubular epithelial cells, and energy metabolism and other functions related to the enzyme activity weakened