论文部分内容阅读
目的 :观察小檗碱对大鼠胃窦平滑肌细胞的收缩作用。方法 :采用Bitar法分离制备大鼠胃窦平滑肌细胞悬液 ;将 3种不同浓度的小檗碱加入细胞悬液中 ,用测微尺观察细胞长度的变化。结果 :与对照组相比 ,终浓度为 0 .5 g·L-1 ,1 g·L-1 的小檗碱可显著促进大鼠胃窦平滑肌细胞收缩 (P <0 .0 5 ) ,并呈剂量 效应关系。结论 :小檗碱对大鼠胃窦平滑肌细胞有直接收缩作用。
Objective: To observe the effect of berberine on contraction of rat gastric antrum smooth muscle cells. METHODS: Rat gastric antrum smooth muscle cell suspension was prepared by Bitar method. Three different concentrations of berberine were added to the cell suspension, and the changes in cell length were observed with a micrometer. RESULTS: Compared with the control group, berberine at a final concentration of 0.5 g·L-1 and 1 g·L-1 significantly promoted the contraction of rat gastric antrum smooth muscle cells (P < 0.05). Dose effect relationship. Conclusion: Berberine has a direct contraction effect on rat gastric antrum smooth muscle cells.