电镜在免疫细胞化学中的应用使神经系统的形态学研究得到了突飞猛进的发展.免疫细胞化学反应阳性结构往往在组织切片中呈散在分机,如果采用一般的电镜包埋技术,势必造成电镜下阳性检出率很低,而且取材部位不准.为了使电镜下观察光镜下所发现的同一细胞或同一条突起,1984年Aldes和Boone介绍了平板包埋法,但此法操作繁琐,特别是聚合后定位切取样品时,组织块易于破碎和飞跳丢失,对此我们进行了探索改 The application of electron microscopy in immunocytochemistry has led to the rapid development of the morphological studies of the nervous system.Immunocytochemical positive structures tend to be scattered in the tissue sections, and if electron microscopy is used, it is bound to result in positive electron microscopy Detection rate is very low, and the material is not allowed to take part in order to make microscopic examination under electron microscopy found the same cell or the same protrusion, Aldes and Boone in 1984 introduced the method of plate embedding, but this method is cumbersome to operate, especially Post-polymerization positioning cut samples, the tissue block is easy to break and fly lost, which we have to explore