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在pH 5.74的HAc-NaAc缓冲溶液中,苋菜红(AAT)与Cu(Ⅱ)相互作用生成新的化合物,引起吸收光谱发生明显变化,522 nm处产生显著的褪色现象,463 nm处出现新的吸收峰。考察了体系的光谱特征、最佳反应条件、影响因素及方法的选择性和灵敏度,发现522 nm处的褪色程度与AAT的浓度在一定范围内具有良好的线性关系,摩尔吸收系数ε为1.46×104L·mol-1·cm-1。由此建立了一种新的测定苋菜红含量的方法,并对AAT与Cu(II)的反应机理进行了探讨。
In the HAc-NaAc buffer solution of pH 5.74, amaranth (AAT) interacted with Cu (Ⅱ) to generate new compounds, causing a significant change in the absorption spectrum, resulting in significant discoloration at 522 nm and a new Absorption peak. The spectral characteristics, the optimum reaction conditions, the influencing factors and the selectivity and sensitivity of the system were investigated. It was found that there was a good linear relationship between the degree of discoloration at 522 nm and the concentration of AAT in a certain range. The molar absorption coefficient ε was 1.46 × 104L · mol-1 · cm-1. A new method for the determination of amaranth content was established, and the reaction mechanism of AAT with Cu (II) was also discussed.