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目的:探讨热休克转录因子1(HSF-1)在高迁移率族蛋白1(HMGB1)所诱导的炎症反应中的作用。方法:不同浓度HMGB1作用于RAW264.7细胞不同时间后,用ELISA法检测细胞上清液TNF-α水平;分别用免疫荧光法与Western blot法检测HMGB1作用后,RAW264.7细胞NF-κB核转移情况与HSF-1表达。观察干扰HSF-1表达后,HMGB1诱导RAW264.7细胞TNF-α表达的变化。结果:HMGB1作用后,RAW264.7细胞分别在4、12 h出现两次TNF-α分泌高峰,且TNF-α的释放量随HMGB1浓度的增加而增加。HMGB1作用后,RAW264.7细胞的NF-κB核转移明显增强,HSF-1表达明显增加(均P<0.05)。干扰HSF-1表达后,HMGB1诱导RAW264.7细胞产生的TNF-α量较未干扰的RAW264.7细胞明显增加(P<0.05)。结论:HMGB1能诱导RAW264.7细胞的炎症反应与HSF-1的表达,但HSF-1可能对HMGB1诱导的炎症反应有负反馈性抑制作用。
AIM: To investigate the role of heat shock transcription factor 1 (HSF-1) in the inflammatory response induced by high mobility group box 1 (HMGB1). Methods: After treated with different concentrations of HMGB1 for different time, RAW264.7 cells were treated with HMGB1 by immunofluorescence and Western blot respectively. The expression of NF-κB in RAW264.7 cells was detected by ELISA. Metastasis and HSF-1 expression. Observe the interference of HSF-1 expression, HMGB1 induced RAW264.7 cells TNF-α expression changes. Results: After HMGB1 treatment, the peak of TNF-α secretion appeared in RAW264.7 cells at 4 and 12 h respectively, and the release of TNF-α increased with the increase of HMGB1 concentration. After HMGB1 treatment, NF-κB nuclear translocation was significantly increased and the expression of HSF-1 was significantly increased in RAW264.7 cells (all P <0.05). After interfering the expression of HSF-1, the amount of TNF-α produced by HMGB1 in RAW264.7 cells was significantly increased compared with that of RAW264.7 cells (P <0.05). Conclusion: HMGB1 can induce the inflammatory response and the expression of HSF-1 in RAW264.7 cells, but HSF-1 may have a negative feedback inhibition effect on the HMGB1-induced inflammatory response.