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目的:优选仙鹤草中总黄酮的提取工艺。方法:以芦丁为对照品,用紫外可见分光光度计测定仙鹤草总黄酮的含量。以总黄酮收率为指标,通过正交试验法优选仙鹤草总黄酮的提取工艺。结果:最佳提取工艺为50%的乙醇,8倍量溶剂,回流提取2次,每次提取1 h。结论:该提取工艺经济、合理、可行,可为仙鹤草总黄酮的提取提供依据。
Objective: To optimize the extraction of total flavonoids from Agrimony. Methods: Rutin was used as reference substance, and the content of total flavonoids in Agrimony was determined by UV-vis spectrophotometer. Taking total flavonoid yield as an index, the extraction process of total flavonoids from Agrimony was optimized by orthogonal test. Results: The best extraction process was 50% ethanol, 8 times the amount of solvent, reflux extraction 2 times, each extraction 1 h. Conclusion: The extraction process is economical, reasonable and feasible, which can provide the basis for the extraction of total flavonoids from Agrimony.