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目的:用RPHPLC法同时测定注射用氨苄氯唑西林的含量。方法:色谱柱μBondapak C18；以0.035 mol·L-1磷酸二氢钾(pH6.5)甲醇(46∶54)为流动相，咖啡因为内标，检测波长为225 nm。结果:氨苄西林、氯唑西林在5～40 μg·ml-1的浓度范围内，线性关系良好；平均回收率分别为99.5%(RSD=1.3%，n=9)，103.1%(RSD=2.0%，n=9)，与USP(ⅩⅩⅢ版)比较无显著性差异(P＞0.05)。结论:该方法简单、快速、准确。
Objective: To determine the content of ampicillin Xylin injection by RP-HPLC simultaneously. Methods: μBondapak C18 column was used as the mobile phase with 0.035 mol·L-1 potassium phosphate monobasic (pH 6.5) -methanol (46:54) as the internal standard. The detection wavelength was 225 nm. Results: Ampicillin and cloxacillin had a good linearity in the range of 5 ~ 40 μg · ml-1. The average recoveries were 99.5% (RSD = 1.3%, n = 9), 103.1% (RSD = 2.0%, n = 9), no significant difference compared with USP (ⅩⅢⅢ) (P> 0.05). Conclusion: The method is simple, rapid and accurate.