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目的:观察不同浓度的琥珀酸对大鼠海马CA1区神经元电压依赖性钙通道(voltage-dependent calcium channels,VDCC)电流的作用,初步探讨琥珀酸对神经元保护的电生理学基础。方法:采用传统全细胞膜片钳技术和制霉菌素(nystatin)穿孔膜片钳技术观察琥珀酸对海马CA1区神经元VDCC电流的影响。结果:不同浓度的琥珀酸(10~(-6)、10~(-5)、10~(-4)、10~(-3)、10~(-2)和10~(-1)mol·L~(-1))在海马CA1区对低电压激活(low-voltage activated,LVA)钙通道电流未见任何影响,而对高电压激活(high-voltage activated,HVA)钙通道电流的抑制呈浓度依赖性。对照组HVA钙电流为580.05±17.32 pA,分别给予10~(-6)、10~(-5)、10~(-4)、10~(-3)、10~(-2)和10~(-1) mol·L~(-1)的琥珀酸后,HVA钙电流依次为563.74±16.65,517.99±15.24,444.66±13.26,405.32±19.11,269.03±9.96和86.41±3.25 pA,同对照组相比差异有统计学意义(n=8,P<0.01)。结论:琥珀酸能浓度依赖性地抑制HVA钙电流,而对LVA钙电流无影响。由此推测琥珀酸可能通过抑制HVA钙电流减少Ca~(2-)内流而影响海马CA1区神经元的兴奋性,从而抑制癫痫的形成,其脑保护作用可能与此有关。
OBJECTIVE: To observe the effects of different concentrations of succinic acid on the voltage-dependent calcium channels (VDCC) currents in the hippocampal CA1 region of rats, and to explore the electrophysiological basis of the protection of neurons by succinic acid. METHODS: The effect of succinic acid on VDCC currents in hippocampal CA1 neurons was observed using traditional whole-cell patch clamp technique and nystatin perforated patch clamp technique. Results: Different concentrations of succinic acid (10~(-6), 10~(-5), 10~(-4), 10~(-3), 10~(-2) and 10~(-1)mol L~(-1)) has no effect on low-voltage activated (LVA) calcium channel currents in the CA1 region of the hippocampus, but inhibits high-voltage activated (HVA) calcium channel currents. In a concentration-dependent manner. In the control group, the HVA calcium current was 580.05±17.32 pA, which was given to 10~(-6), 10~(-5), 10~(-4), 10~(-3), 10~(-2) and 10~ After (-1) mol·L -1 succinic acid, HVA calcium current was 563.74±16.65,517.99±15.24,444.66±13.26,405.32±19.11,269.03±9.96 and 86.41±3.25 pA, respectively. The difference was statistically significant (n=8, P<0.01). Conclusion: Succinic acid can inhibit the calcium current of HVA in a concentration-dependent manner without affecting the calcium current of LVA. It is speculated that succinic acid may affect the excitability of hippocampal CA1 neurons by inhibiting the calcium current of HVA to reduce Ca~(2-) influx, thereby inhibiting the formation of epilepsy, and its brain protection may be related to this.