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研究蛋白酶体抑制剂硼替佐米(bortezomi)对白血病细胞株HL-60细胞增殖、凋亡及对RelA、Apollon基因表达的影响。将不同浓度的硼替佐米作用于HL-60细胞,采用水溶性四唑盐光吸收法(WST-1)检测细胞增殖活性,瑞氏(Wrightsstain)染色法观察细胞形态学变化,流式细胞术检测细胞凋亡,RT-PCR法检测RelA、Apollon mRNA表达,SP免疫组化法检测RelA、Apollon蛋白表达。不同浓度的硼替佐米分别作用于HL-60细胞24h,均有增殖抑制、诱导凋亡的作用,与空白对照组比较,差异具有统计学意义(P<0.01);各实验组之间相互比较,也有显著差异(P<0.05)。不同浓度的硼替佐米均能下调RelA、Apollon基因和蛋白的表达,与空白对照组比较,差异具有统计学意义(P<0.01)。硼替佐米能抑制HL-60细胞增殖,并下调RelA、Apollon的表达而诱导细胞凋亡;RelA、Apollon表达呈正相关,RelA可能参与指导了Apollon的表达。
To investigate the effects of bortezomi, a proteasome inhibitor, on the proliferation, apoptosis and the expression of RelA and Apollon in leukemia cell line HL-60. Bortezomib at different concentrations was applied to HL-60 cells. Cell proliferation activity was measured by water-soluble tetrazolium salt (WST-1) absorption assay. Cell morphology was observed by Wright’s stain. Flow cytometry Cell apoptosis was detected by flow cytometry. The expression of RelA and Apollon mRNA was detected by RT-PCR. The expression of RelA and Apollon protein was detected by SP immunohistochemistry. Bortezomib at different concentrations were treated with HL-60 cells for 24 h, respectively. Both of them had the effects of inhibiting proliferation and inducing apoptosis. Compared with the blank control group, the difference was statistically significant (P <0.01); comparing with each other , There are also significant differences (P <0.05). Different concentrations of bortezomib could down-regulate the expression of RelA, Apollon gene and protein, which had statistical significance compared with the blank control group (P <0.01). Bortezomib could inhibit the proliferation of HL-60 cells and down-regulate the expression of RelA and Apollon to induce apoptosis. RelA and Apollon expression were positively correlated, and RelA may be involved in guiding Apollon expression.