为了检测贝类标本中的甲型肝炎病毒，我们建立了敏感特异的免疫捕获—逆转录—聚合酶链反应技术（ＩＣ－ＲＴ－ＰＣＲ）。应用该技术检测不同地区采集的贝类标本中的ＨＡＶ－ＲＮＡ后发现：４６份贝类标本有２９份为阳性，检出率达６３０４％（２９／４６），其中毛蚶为６３１６％（１２／１９），文蛤为９０００％（９／１０），蚶子为５４５５％（６／１１），说明各种贝类有不同程度的污染；南部、中部地区贝类标本中的ＨＡＶ－ＲＮＡ检出率较高，北部地区１０份标本仅检出１份阳性，这与当地甲肝发病率较低一致。 To detect Hepatitis A virus in shellfish specimens, we established a sensitive and specific immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR). Using this technique to detect HAV-RNA in shellfish samples collected from different areas, 29 samples of 46 shellfish samples were positive, with a detection rate of 6304% (29/46), of which 6316 (12/19), clam 9000% (9/10), clam 5455% (6/11), which showed that shellfish had different degrees of pollution. Shellfish specimens from southern and central China In the detection rate of HAV-RNA is higher, 10 samples in the northern region only detected a positive, which is consistent with the lower incidence of local hepatitis A.