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目的研究沉默信息调节因子2同源蛋白1(SIRT1)在甲状腺乳头状癌组织中的表达及其临床意义。方法采用免疫组织化学法和组织芯片技术检测88例甲状腺乳头状癌组织中SIRT1的表达,分析SIRT1蛋白的表达情况及其与甲状腺乳头状癌临床病理特征的关系。结果SIRT1阳性染色主要集中在细胞核中,呈黄色或者棕色颗粒。根据免疫组织化学染色评分,88例甲状腺乳头状癌组织中SIRT1阳性率为48.9%。χ~2检验结果显示,SIRT1表达与颈部淋巴结转移、TNM分期显著相关(P<0.05),SIRT1表达与年龄、性别、肿瘤大小、癌灶多少、甲状腺包膜浸润不相关(P>0.05)。Logistic回归分析显示,SIRT1阳性是甲状腺乳头状癌颈部淋巴结转移的独立危险因素(P<0.05)。结论 SIRT1可能参与甲状腺乳头状癌的发生发展和颈部淋巴结转移,并可能成为甲状腺乳头状癌颈部淋巴结转移的治疗靶点。
Objective To study the expression of SIRT1 in thyroid papillary carcinoma and its clinical significance. Methods Immunohistochemistry and tissue microarray were used to detect the expression of SIRT1 in 88 cases of thyroid papillary carcinoma. The relationship between the expression of SIRT1 protein and the clinicopathological features of papillary thyroid carcinoma was analyzed. Results SIRT1 positive staining mainly in the nucleus, yellow or brown particles. According to the immunohistochemical staining score, the positive rate of SIRT1 in 88 cases of papillary thyroid carcinoma was 48.9%. The results of χ ~ 2 test showed that the expression of SIRT1 was significantly correlated with lymph node metastasis and TNM stage in cervical lymph nodes (P <0.05). The expression of SIRT1 was not related to age, gender, tumor size, tumor size and thyroid capsule infiltration (P> 0.05) . Logistic regression analysis showed that SIRT1 positive was an independent risk factor for cervical lymph node metastasis in papillary thyroid carcinoma (P <0.05). Conclusion SIRT1 may be involved in the occurrence and development of papillary thyroid carcinoma and cervical lymph node metastasis, and may be a therapeutic target for cervical lymph node metastasis of papillary thyroid carcinoma.