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目的:观察鼠神经生长因子(mouse nerve growth factor，mNGF)对创伤性脑损伤(traumatic brain injury，TBI)合并海水淹溺(seawater drowning，SWD)大鼠脑组织含水量、海马组织n β-淀粉样前体蛋白(n β-amyloid precursor protein，β-APP)及神经丝蛋白轻链多肽(neurofilament light polypeptide，NF-L)表达的影响，评估其干预效果。n 方法:60只SD大鼠随机分为假手术(Sham)组(n n＝12)、TBI＋SWD组(n n＝24)、mNGF组(n n＝24)。采用Marmarou颅脑致伤法及气管内泵入海水法建立大鼠模型。mNGF组于伤后立即腹腔注射1 ml mNGF(3 μg/kg)，Sham组、TBI＋SWD组腹腔注射等量生理盐水。采用干湿质量法、苏木精-伊红(HE)染色法和免疫组织化学(IHC)染色法分别检测大鼠脑组织含水量及海马组织病理变化、β-APP和NF-L的表达。n 结果:mNGF组与TBI＋SWD组脑组织含水量相似，差异无统计学意义(n P>0.05)；与TBI＋SWD组比较，mNGF组海马组织中β-APP及NF-L表达减弱。n 结论:mNGF可减弱β-APP及NF-L表达，对TBI＋SWD大鼠神经细胞具有保护作用。“,”Objective:To evaluate the effects of mouse nerve growth factor (mNGF) on the brain water content and the expressions of n β-amyloid precursor protein (β-APP) and neurofilament light polypeptide (NF-L) in rats with traumatic brain injury (TBI) complicated by seawater drowning (SWD).n Methods:A total of 60 male rats were randomly divided into three groups: Sham group (n n=12), TBI+ SWD group (n n=24), and mNGF group (n n=24). The rat models were created on the basis of Marmarou′s weight-drop TBI modeling and instilling seawater into the trachea by syringe pump. The mNGF group was administered mNGF intraperitoneally; while the Sham group and the TBI+ SWD group were injected with an equivalent amount of normal saline. The brain water content was measured by using the dry-wet weight method. The pathological changes of the hippocampal tissue and the expressions of β-APP and NF-L were observed through hematoxylin-eosin (HE) staining and immunohistochemical (IHC) staining.n Results:Brain water content in the mNGF group was similar to that in the TBI+ SWD group (n P>0.05). Compared with the TBI+ SWD group, the expressions of β-APP and NF-L in the hippocampal tissue of the mNGF group were significantly reduced.n Conclusion:mNGF can reduce the expressions of β-APP and NF-L, and protect the neurons of the rats after TBI+ SWD.