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在自然界广泛地存在着联合固氮作用及其相应的微生物,许多研究工作者正在对此进行广泛而深入的研究。我们对东北地区几种主要禾谷类大田作物玉米、高粱、小麦和谷子等进行了作物与微生物联合共生固氮作用的研究。 一、材料和方法 (一)培养基 1.H培养基成分 (克/立升):K_2HPO_4,0.8; KH_2PO_4,0.2;MgSO_4·7H_2O,0.2;NaCl,0.1;CaCl_2,0.02;FeCl_3,0.01;酵母片,0.2;苹果酸,3.0;葡萄糖,10.0,微量元素液,1.0ml;蒸镏水,1000ml,用NaOH调PH至6.0～6.5。
There are a wide range of combined nitrogen fixation and its corresponding microorganisms in nature, and many researchers are conducting extensive and in-depth research. We studied the symbiotic nitrogen fixation of crops and microorganisms on some major cereal field crops such as corn, sorghum, wheat and millet in Northeast China. Materials and methods (a) Medium 1.H medium composition (g / liter): K 2 HPO 4, 0.8; KH 2 PO 4, 0.2; MgSO 4 4.7H 2 O, 0.2; NaCl, 0.1; CaCl 2, 0.02; FeCl 3, Tablets, 0.2; malic acid, 3.0; glucose, 10.0, trace element solution, 1.0ml; distilled water, 1000ml, adjusted to 6.0-6.5 with NaOH.