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目的:观察三拗汤对哮喘模型大鼠转化生长因子β-1及神经生长因子表达的影响,并探究其可能的机制。方法:40只SD大鼠均分为正常对照组、哮喘模型组、布地奈德组(2.5×10-4g/kg)及三拗汤组(39 g/kg)。以鸡卵清蛋白致敏加激发法复制哮喘模型,最终测量气道壁厚度及气道平滑肌厚度;酶联免疫吸附法检测支气管肺泡灌洗液及血清中转化生长因子β-1的浓度;免疫组化法检测神经生长因子表达水平。结果:与正常对照组相比,哮喘模型组气道壁厚度及气道平滑肌厚度均增加(15.08±0.41、10.52±0.28 vs 43.65±1.25、32.64±1.18,P<0.01),支气管肺泡灌洗液及血清中转化生长因子β-1的浓度上升(58.75±1.96、98.13±4.44 vs 115.88±3.47、195.89±6.18,P<0.01),肺组织中神经生长因子表达水平升高(97.15±3.05 vs 165.87±5.12,P<0.01);与哮喘模型组比较,布地奈德组(2.5×10-4g/kg)、三拗汤组(39 g/kg)中气道壁厚度及气道平滑肌厚度均减小(43.65±1.25、32.64±1.18 vs 22.15±0.62、16.43±0.45;21.47±0.95、16.10±0.49,P<0.01),支气管肺泡灌洗液及血清中转化生长因子β-1的浓度下降(115.88±3.47、195.89±6.18 vs 84.05±2.86、138.80±4.90;79.62±3.26、141.03±5.87,P<0.01),肺组织中神经生长因子表达水平降低(165.87±5.12 vs 117.55±3.72;114.35±3.43,P<0.01);布地奈德组、三拗汤组上述指标间比较,差异无统计学意义。结论:三拗汤对减轻哮喘大鼠气道重塑具有明显的影响,其机制可能与降低TGFβ-1浓度及下调肺组织中NGF蛋白表达水平相关。
Objective: To observe the effect of San’ao Decoction on the expression of transforming growth factor β-1 and nerve growth factor in asthmatic rats, and to explore its possible mechanism. Methods: Forty SD rats were divided into normal control group, asthma model group, budesonide group (2.5 × 10-4g / kg) and Sanao decoction group (39g / kg). Asthmatic model was induced by chicken ovalbumin sensitization plus stimulation, and the thickness of airway wall and the thickness of airway smooth muscle were measured finally. The concentrations of TGF-β1 in bronchoalveolar lavage fluid and serum were detected by enzyme-linked immunosorbent assay The histochemical method was used to detect the expression of nerve growth factor. Results: Compared with the normal control group, the airway wall thickness and airway smooth muscle thickness were increased in asthmatic model group (15.08 ± 0.41, 10.52 ± 0.28 vs 43.65 ± 1.25, 32.64 ± 1.18, P <0.01), and bronchoalveolar lavage fluid (58.75 ± 1.96, 98.13 ± 4.44 vs 115.88 ± 3.47, 195.89 ± 6.18, P <0.01), the expression of NGF in serum increased (97.15 ± 3.05 vs 165.87 ± 5.12, P <0.01). Compared with asthma model group, airway wall thickness and airway smooth muscle thickness in budesonide group (2.5 × 10-4g / kg) and Sanao decoction group (39g / kg) (43.65 ± 1.25,32.64 ± 1.18 vs 22.15 ± 0.62,16.43 ± 0.45; 21.47 ± 0.95,16.10 ± 0.49, P <0.01). The concentrations of TGF-β1 in bronchoalveolar lavage fluid and serum were decreased (115.88 ± 3.47, 195.89 ± 6.18 vs 84.05 ± 2.86, 138.80 ± 4.90, 79.62 ± 3.26, 141.03 ± 5.87, P <0.01). The expression of NGF in lung tissue was decreased (165.87 ± 5.12 vs 117.55 ± 3.72; 114.35 ± 3.43, P <0.01). The budesonide group and Sanao decoction group showed no significant difference between the above indexes. Conclusion: Sanao Decoction can significantly reduce the airway remodeling in asthmatic rats. The mechanism may be related to decreasing the concentration of TGFβ-1 and down-regulating the expression of NGF in lung tissue.