综述了现代二维核磁共振技术测定ＤＮＡ结构的进展、方法及其原理和一般步骤．从ＤＮＡ的基本结构出发，应用二维ＮＯＥ谱及其它ＮＭＲ方法，获得大量的质子间距离、键长和键角数据，再经距离几何学、分子动力学等数据处理手段，可获知ＤＮＡ在溶液中的三维结构． The progress, method, principle and general steps of measuring DNA structure by modern two-dimensional nuclear magnetic resonance technique are reviewed. Starting from the basic structure of DNA, two-dimensional NOE spectra and other NMR methods were used to obtain a large number of proton distance, bond length and bond angle data, and then by distance geometry, molecular dynamics and other data processing means, we can know DNA in solution In the three-dimensional structure.