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背景:CDX2与肠源性肿瘤的发生密切相关,而p21表达减低或缺失可能是结直肠癌发生、发展中的一个普遍事件。目的:探讨结直肠癌中CDX2、p21~(H-ras)、p21~(WAF1)表达的相关性和临床病理意义。方法:收集45例临床病理资料完整的结直肠癌手术切除标本,以免疫组化方法检测癌组织和相应癌旁组织中的CDX2、p21~(H-ras)、p21~(WAF1)蛋白表达。结果:结直肠癌组织中CDX2、p21~(H-ras)、p21~(WAF1)阳性率分别为86.7%、68.9%和35.6%,相应癌旁组织中阳性率分别为100.0%、37.8%和51.1%,CDX2和p21~(H-ras)在癌组织和癌旁组织中的阳性率差异有统计学意义(P=0.026和P=0.006);半定量分析结果显示CDX2和p21~(WAF1)在癌组织和癌旁组织中的表达水平差异有统计学意义(P=0.007和P=0.005)。癌组织中CDX2与p21~(H-ras)的表达存在相关性(r_s=0.501,P=0.000)。Dukes A、B期患者癌组织p21~(WAF1)阳性率显著高于C、D期患者(P=0.016),有淋巴结转移者p21~(WAF1)阳性率显著低于无淋巴结转移者(P=0.048),CDX2、p21~(H-ras)的表达与结直肠癌各临床病理特征均无相关性。结论:CDX2、p21~(H-ras)、p21~(WAF1)表达改变可能与结直肠癌的发生、发展相关。
BACKGROUND: CDX2 is closely related to the occurrence of intestinal tumors, and the decrease or absence of p21 expression may be a common event in the development and progression of colorectal cancer. Objective: To investigate the correlation and clinicopathological significance of the expression of CDX2, p21 H-ras and p21 WAF1 in colorectal cancer. Methods: Forty-five cases of complete colorectal cancer resection specimens were collected. The expression of CDX2, p21 H-ras, and p21 WAF1 proteins in cancer tissues and corresponding paracancerous tissues were detected by immunohistochemistry. RESULTS: The positive rates of CDX2, p21 H-ras and p21 WAF1 in colorectal cancer tissues were 86.7%, 68.9% and 35.6%, respectively, and the corresponding positive rates in adjacent tissues were 100.0% and 37.8% respectively. In 51.1%, the positive rates of CDX2 and p21 H-ras in cancer tissues and adjacent tissues were statistically significant (P=0.026 and P=0.006); semi-quantitative analysis showed that CDX2 and p21~(WAF1) There was a statistically significant difference in the expression levels between cancer tissues and adjacent tissues (P=0.007 and P=0.005). There was a correlation between the expression of CDX2 and p21 H-ras in cancer tissues (r_s=0.501, P=0.000). The positive rate of p21~(WAF1) was significantly higher in Dukes A and B than in C and D (P=0.016). The positive rate of p21~(WAF1) in lymph node metastasis was significantly lower than in those without lymph node metastasis (P= There was no correlation between the expression of CDX2 and p21~(H-ras) and clinicopathological features of colorectal cancer. Conclusion: The changes of the expression of CDX2, p21 H-ras and p21 WAF1 may be related to the occurrence and development of colorectal cancer.