The soil nearby a gas station was collected, from which we isolated a series of strains by means of enrichment and screening technigues. The most effective strain for the degradation of toluene, i.e. , JYZ0315was screened by the further mutation of the above strains. The degradation capacity experiment was carried out in simulated groundwater. Under the optimal conditions, the degradation rate could reach 93. 5%. During the extend application experiments with a percolation tank, the degradation of toluene reached 87.3% in 11 d and 95.2% in 15 d, which was close to that of the microenvironment, and this proves that JYZ0315 is one of the predominant strains to degrade toluene in groundwater.