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目的探讨移植骨髓间充质干细胞(MSC)对肺气肿大鼠慢性炎症的调控作用。方法烟熏法复制大鼠肺气肿模型。携带绿色荧光蛋白(GFP)的慢病毒转染MSC,烟熏大鼠肺内输入转染的MSC(n=4),小动物活体成像系统观察MSC的分布。36只SD大鼠随机分为对照组、肺气肿组及MSC干预组,评估支气管肺泡灌洗液(BALF)中细胞总数和分类计数,双抗体夹心酶联免疫吸附测定法(ELISA)检测BALF、血清中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)水平,比色法检测肺组织丙二醛(MDA)含量,测量平均内衬间隔(MLI)评估肺气肿改变。结果肺内输入MSC 4周后,不同肺叶仍可见存活的MSC。与肺气肿组相比,MSC干预组BALF中细胞总数、血清和BALF中TNF-α及IL-1β水平、肺组织中MDA含量和MLI均明显减低,差异有统计学意义(P<0.01)。结论 MSC降低肺气肿大鼠气道及全身炎症介质TNF-α和IL-1β的表达,减轻气道炎症和氧化应激水平,对肺气肿有明显的治疗作用。
Objective To investigate the regulatory effect of transplanted bone marrow mesenchymal stem cells (MSCs) on chronic inflammation in rats with emphysema. Methods The rat model of emphysema was duplicated by smoking. MSCs were transfected with lentivirus carrying green fluorescent protein (GFP), and transfected MSCs were transfected into the lungs of smoked rats (n = 4). The distribution of MSCs was observed by living animal imaging system. Thirty-six Sprague-Dawley rats were randomly divided into control group, emphysema group and MSC intervention group to evaluate the total number of cells and the number of bronchoalveolar lavage fluid (BALF). BALF was detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) (TNF-α) and interleukin-1β (IL-1β) were detected by enzyme-linked immunosorbent assay (ELISA). Malondialdehyde (MDA) change. Results After MSC was infused into the lungs for 4 weeks, surviving MSCs could still be seen in different lung lobes. Compared with the emphysema group, the total number of BALF cells, the level of TNF-α and IL-1β in serum and BALF, the content of MDA and the content of MLI in lung tissue of MSC-treated group were significantly lower than those in emphysematous group (P <0.01) . Conclusion MSC can reduce the expression of TNF-α and IL-1β in airway and systemic inflammatory mediators of emphysema rats, reduce airway inflammation and oxidative stress, and have a significant therapeutic effect on emphysema.