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1材料与方法1.1材料除电子显微镜样品染色常规用的材料外,另备平板包埋硅胶板若干块(以下简称硅胶板),无齿专用镊(夹取铜网用),盛双蒸馏水的25ml小烧杯若干(一般为6个),遮光用的盒盖。将经4%冰醋酸溶液浸泡3-6小时的硅胶包埋板及橡胶头吸管反复用双蒸水冲洗干净,以去除残留的冰醋酸,放置温箱中烘干备用。1.2方法以橡皮头吸管吸取适量的铀染液,按所需染色的铜网数,在相应效目包埋板路中逐个滴入染液(尽量使染液形成一个小液珠),将待染色的铜网(的标本的一
1 Materials and methods 1.1 Materials In addition to electron microscopy sample staining conventional materials, the other plate embedded a few pieces of silica gel plate (hereinafter referred to as silica gel plate), toothless tweezers (with copper net), Sheng double distilled water 25ml A small number of beaker (usually 6), cover with light. The 4% glacial acetic acid solution soaked 3-6 hours silica gel embedded plate and rubber head pipette repeatedly rinsed with double distilled water to remove residual glacial acetic acid, placed in the oven drying reserve. 1.2 Methods Eraser pipette appropriate amount of uranium dye solution, according to the desired number of copper staining, embedded in the corresponding effect buried plate one by one into the dye bath (as far as possible to form a liquid dye beads), will be Dyed copper mesh (one of the specimens