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目的对人乳头瘤病毒58型(HPV58)主要结构蛋白(L1)的B细胞表位进行预测和分析。方法利用生物信息学软件EXPASY在线分析HPV58型L1蛋白的二级结构及生物学特性,包括跨膜趋势、表面可及性、抗原性、亲水性,溶性等,从而对其B细胞表位进行综合分析和预测。结果通过亲水性参数,Zimmcrman极性,柔韧性参数,表面可及性及抗原性进行综合分析,预测人乳头瘤病毒58型L1蛋白的B细胞表位可能位于其N端的29-37,43,46-58,64-67,74,79-88,93,100-101,103-125,133-139,149,151-180,187-213,220-213,235,238-247,252-271,277-282,286-309,320-323,327-338,341-348,351,353-368,373-396,408,427,419-425,433-444,453-468,470,475-486,488-490,493-494,497-524肽段区域;进一步用Protien Plast在线同源匹配分析,79-88、190-216、373-396、453-468、497-524肽段区段为可能的B细胞表位。结论用多参数预测分析HPV58型L1蛋白可能的B细胞表位,为进一步研究高危型58型L1蛋白的生物学特性提供了理论基础。
Objective To predict and analyze the B cell epitopes of human papillomavirus type 58 (HPV58) major structural protein (L1). Methods The bio-informatics software EXPASY was used to analyze the secondary structure and biological characteristics of HPV58 L1 protein including transmembrane tendency, surface accessibility, antigenicity, hydrophilicity, solubility and so on. Comprehensive analysis and forecast. Results The comprehensive analysis of hydrophilicity parameters, Zimmcrman polarity, flexibility parameters, surface accessibility and antigenicity predicted that the B cell epitope of human papillomavirus Type 58 L1 protein might be located at the N-terminal 29-37,43 , 46-58,64-67,74,79-88,93,100-101,103-125,133-139,149,151-180,187-213,220-213,235,238-247,252-271,277-282,286-309,320-323,327-338,341-348,351,353-368,373-396,408,427,419-425,433-444,453 -468, 470, 475-486, 488-490, 493-494, 497-524 peptide regions; further analysis using Protien Plast online homologous matching, 79-88, 190-216, 373-396, 453-468, 497-524 peptide segments are possible B cell epitope. Conclusion The multi-parameter prediction of possible B cell epitopes of HPV58 L1 protein provides a theoretical basis for further study on the biological characteristics of high-risk type 58 L1 protein.