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本文报道作者从感染的组织培养液中制备HA抗原,并用丙酮提取和超声处理法提高HFRS HA灭活抗原滴度,研究了其在HI试验中的应用。作者将HFRS相关病毒B-1株感染VeroE6细胞,取1～2周的培养液(F)低速离心,除去细胞碎片。上清液经25000转/分离心后,沉淀物(P-2)用pH9牛白蛋白-硼酸盐溶液(BABS)制成悬液,再用丙酮和丙酮-乙醚抽提得A或AE,最后用超声处理3或10分钟制成AS-3’或AS-10’。用鹅红细胞测定不同抗原的HA滴度,结果发现感染组织培养液F的滴度只有1:16,4℃过夜后滴度<1:2;P-2滴度上升为1:1024,电镜观察到其中皆为
This article reports the preparation of HA antigen from infected tissue culture fluid and the use of acetone extraction and sonication to increase the HFRS HA inactivated antigen titers. The authors infected Vero E6 cells with HFRS-associated virus B-1 strain and centrifuged at low speed for 1 to 2 weeks (F) to remove cell debris. After the supernatant was centrifuged at 25000 rpm, the precipitate (P-2) was suspended in pH 9 bovine albumin-borate solution (BABS), and then A or AE was extracted with acetone and acetone- Finally, sonicated for 3 or 10 minutes to make AS-3 ’or AS-10’. The goat erythrocytes were used to measure the HA titers of different antigens. The results showed that the titer of infected tissue culture fluid F was only 1: 16, the titer was <1: 2 after overnight at 4 ℃, the titer of P-2 was 1: 1024, To all of them