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目的 通过三年的监测 ,发现E .coliO1 5 7∶H7感染性腹泻的分布特征、临床特点、家畜、家禽的带菌状况及外环境污染程度。方法 用O1 5 7特异性筛查方法进行病原体分离培养 ,应用分子生学、微生物学、生化学技术进行病人、家畜、家禽的病原学分离、培养、毒素因子测定。结果 2 0 0 0年— 2 0 0 2年从 2 5 4 1份标本中检出E .coliO1 5 7∶H71 99株 ,其中 76株具有毒素基因 ;显示有 5个毒素因子组合型。结论 E .coliO1 5 7∶H7是引起发病的主要致病菌 ,发病率的高低与家畜家禽的带菌相一致
OBJECTIVE Through three years of surveillance, it was found that E.coliO1 5 7:H7 infectious diarrhea distribution characteristics, clinical features, livestock and poultry carrier status and environmental pollution levels. Methods The specific screening method of O157 was used to isolate and culture pathogens. The pathogenic isolation, culture and toxin factors of patients, livestock and poultry were determined by molecular biology, microbiology and biochemical techniques. Results From 2000 to 2002, 99 strains of E.coliO1 5 7:71 were detected, of which 76 strains had toxin genes. Five combinations of toxin factors were found. Conclusion E.coliO1 5 7:H7 is the main pathogenic bacteria, and the incidence is consistent with the carriage of livestock poultry