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本文报道了人血清中氟卡胺的HPLC立体测定方法。氟卡胺标准贮备液及内标溶液(2,5-二乙氧基-N-(2-哌啶基甲基)苯甲酰胺盐酸盐)分别由10mg外消旋醋酸盐溶于1000ml去离子水中(10μg/ml),工作标准液则将贮备液稀释10倍而得。在试管中放入1.0 ml血浆,然后加入50μl内标溶液(50 ng),1 ml Tris盐酸(2.0 M,pH 8.5)缓冲液和过量(3 g)氯化钠。NaCl极度溶解后,用5 ml氯丁烷:2-丙醇(95∶5v/v)旋涡提取30秒钟。在室温以每分钟3000
This paper reports the HPLC determination of flecainide in human serum. Flecainide standard stock solution and internal standard solution (2,5-diethoxy-N- (2-piperidinylmethyl) benzamide hydrochloride) were each dissolved in 1000 ml of 10 mg of racemic acetate Deionized water (10μg / ml), the working standard will dilute the stock solution obtained 10 times. Add 1.0 ml of plasma to the tube, then add 50 μl of the internal standard solution (50 ng), 1 ml of Tris-HCl (2.0 M, pH 8.5) buffer and excess (3 g) of sodium chloride. After the NaCl is extremely soluble, it is swirled with 5 ml of chlorobutane: 2-propanol (95: 5 v / v) for 30 seconds. 3000 per minute at room temperature