论文部分内容阅读
目的去卵巢大鼠发生急性心肌梗死前后,应用雌激素干预,观察其外周血干细胞的变化及其对心功能的影响。方法将30只SD雌性大鼠分为正常对照组、急性心肌梗死组、去卵巢心肌梗死组、去卵巢雌激素替代组及去卵巢雌激素治疗组。用流式细胞术检测各组CD90+细胞的百分率;用POWERLAB4.12系统测量左室收缩末压(LVESP)、左室舒张末压(LVEDP)、+dp/dtmax及-dp/dtmax。结果去卵巢心肌梗死组外周血CD90+细胞的百分率显著低于急性心肌塞组及去卵巢雌激素替代组(P<0.01);而去卵巢雌激素替代组心梗后第1天外周血CD90+细胞的百分率即显著升高(P<0.05),第3天达峰值,且数值较高。去卵巢雌激素治疗组与去卵巢心肌梗死组相比较,仅在心梗后7d外周血CD90+细胞的百分率才开始升高;但与去卵巢雌激素替代组相比,心梗术后7d内各时点均较低。各组LVESP均有所下降,以急性心肌梗死组为著;而LVEDP均有升高,以去卵巢心肌梗死组及去卵巢雌激素治疗组最明显(P<0.01)。结论去卵巢大鼠在心肌梗死之前应用雌激素替代治疗,外周血CD90+细胞的百分率较高,且心功能明显优于去卵巢大鼠在心肌梗死后应用雌激素替代治疗。
Objective To study the changes of peripheral blood stem cells and its influence on cardiac function before and after the onset of acute myocardial infarction in ovariectomized rats. Methods Thirty SD female rats were divided into normal control group, acute myocardial infarction group, ovariectomized myocardial infarction group, ovariectomized estrogen replacement group and ovariectomized estrogen treatment group. The percentage of CD90 + cells in each group was detected by flow cytometry. Left ventricular end-systolic pressure (LVESP), left ventricular end-diastolic pressure (LVEDP), + dp / dtmax and -dp / dtmax were measured by POWERLAB4.12 system. Results The percentage of CD90 + cells in peripheral blood in ovariectomy group was significantly lower than that in acute myocardial infarction group and ovariectomized estrogen replacement group (P <0.01). In ovariectomized estrogen replacement group, the percentage of CD90 + The percentage was significantly higher (P <0.05), peaked on the third day, and the value was higher. Ovarian estrogen treatment group and ovariectomy myocardial infarction group, only in 7d after myocardial infarction CD90 + cells began to increase the percentage of peripheral blood; but compared with ovariectomized estrogen replacement group, within 7d after myocardial infarction Time is lower. LVESP decreased in all groups, with acute myocardial infarction as the group; and LVEDP increased, with ovarian myocardial infarction and ovariectomized estrogen treatment group was the most significant (P <0.01). Conclusions Ovariectomized rats should be treated with estrogen replacement therapy before myocardial infarction. The percentage of CD90 + cells in peripheral blood is higher than that in ovariectomized rats. The cardiac function of ovariectomized rats is better than that of ovariectomized rats.