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目的:检测新疆哈萨克族食管鳞癌患者中人乳头状瘤病毒(HPV)DNA的总感染率,通过统计学分析,探讨HPV感染与新疆哈萨克族食管鳞癌的相关性。方法:采用聚合酶链反应(PCR)技术检测了318例新疆哈萨克族食管鳞癌(ESCC)石蜡包埋组织中HPVDNA的感染情况,其中117例用直接裂解法制备样本DNA,201例用酚-氯仿-异戊醇提取DNA;PCR产物双向测序,测序结果应用BLAST在线分析,统计处理采用χ2检验,分析HPV与新疆哈萨克族食管鳞癌发生的相关性。结果:在新疆哈萨克族食管鳞癌组织中,HPVDNA总检出率为64.5%(205/318),其中直接裂解法组HPVDNA检出率为82.9%(97/117),酚-氯仿-异戊醇提取DNA组HPVDNA检出率为53.7%(108/201),二者之间差异有统计学意义(P<0.05)。按照病理分化程度对哈萨克族ESCC样本DNA中HPV总感染率进行分析发现,高分化ESCC中HPV阳性率为63.2%,中分化ESCC中HPV阳性率为69.8%,低分化ESCC中HPV阳性率为50.0%,三者之间差异无统计学意(P>0.05)。结论:HPVDNA感染与新疆哈萨克族食管鳞癌存在相关性,可能是新疆哈萨克族食管癌发生的重要因素之一。
Objective: To detect the total infection rate of human papillomavirus (HPV) DNA in Xinjiang Kazak esophageal squamous cell carcinoma patients and to investigate the correlation between HPV infection and esophageal squamous cell carcinoma of Xinjiang Kazakh by statistical analysis. Methods: HPVDNA infection in paraffin-embedded tissues of 318 Xinjiang Kazakh esophageal squamous cell carcinoma (ESCC) samples was detected by polymerase chain reaction (PCR) technique. Of 117 cases, DNA samples were prepared by direct lytic method and 201 cases were treated with phenol- Chloroform-isoamyl alcohol. The PCR products were bi-directionally sequenced. The sequencing results were analyzed by BLAST online. Statistical analysis was performed using χ2 test to analyze the correlation between HPV and esophageal squamous cell carcinoma in Kazak of Xinjiang. Results: The total detection rate of HPVDNA was 64.5% (205/318) in Xinjiang Kazakh esophageal squamous cell carcinoma. The positive rate of HPVDNA in direct esophageal squamous cell carcinoma was 82.9% (97/117), phenol-chloroform-isoprene The detection rate of HPVDNA in DNA extraction group was 53.7% (108/201), the difference was statistically significant (P <0.05). According to the degree of pathological differentiation of Kazakh ESCC sample DNA in the total infection rate of HPV analysis found that highly differentiated ESCC HPV positive rate was 63.2%, moderately differentiated ESCC HPV positive rate was 69.8%, poorly differentiated ESCC HPV positive rate was 50.0 %, No significant difference between the three (P> 0.05). Conclusion: There is a correlation between HPVDNA infection and esophageal squamous cell carcinoma of Kazak in Xinjiang, which may be one of the important factors for the occurrence of esophageal carcinoma in Kazak of Xinjiang.