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人肺癌细胞株 SPC-A1加入全反式维甲酸(ATRA)使其终浓度为10μmol/L。培养7d 后处理组和对照组分别进行 MTT、光镜、电镜及流式细胞仪检测发现处理组细胞增殖减慢,细胞聚集成片,胞浆中空泡增多,核浆比例下降,无明显染色体固缩及凋亡小体。细胞周期动力学分析发现处理后的细胞生长周期向 G_(0/1)期聚集,S 期和 G_2+M 下降。提示 ATRA 对体外培养的人肺癌 SPC-A1株有一定的生长抑制和诱导分化作用,但凋亡作用不明显。
Human lung cancer cell line SPC-A1 was added with all-trans retinoic acid (ATRA) to a final concentration of 10 μmol/L. After 7 days of culture, MTT, light microscopy, electron microscopy, and flow cytometry were performed on the treated and control groups, respectively. The proliferation of the treated cells was slowed down, the cells aggregated into slices, the number of vacuoles in the cytoplasm increased, the proportion of nuclear plasma decreased, and no obvious chromosome solidification was observed. Reducing apoptotic bodies. Cell cycle kinetic analysis revealed that the cell growth cycle after treatment was aggregated to the G_(0/1) phase and the S phase and G_2+M decreased. It is suggested that ATRA has a certain growth inhibition and differentiation effect on human lung cancer SPC-A1 strain cultured in vitro, but the apoptosis is not obvious.