论文部分内容阅读
本文对南昌霉素 A 效价的生物和化学的测定方法分别进行了研究.生物测定方面筛选到对南昌霉素 A 的敏感菌、最适培养基及 pH 缓冲液.在5～50微克/毫升浓度范围内,敏感菌的抑菌圈直径与南昌霉素 A 的浓度的对数呈线性关系.化学测定方面探索到使南昌霉素 A 乙醇溶液显色的显色剂,摸清了最适显色条件和待测样品的处理方法.南昌霉素 A 与显色剂作用后的溶液显红色,颜色稳定,特异性强,显色后的溶液在721分光光度计上的最大吸收波长为520毫微米,在5～80微克/毫升浓度范围内其吸光度与南昌霉素 A 的浓度成正比,符合朗伯(Lambert)-比耳(Beer)定律。采用南昌霉素 A 标准品加入法和标准曲线法两种方法分别测定同一发酵液中南昌霉素 A 的含量,所获得的测定结果非常接近,表明本方法的置信度达到99%,样品效价测定的全过程可在1小时内完成.本研究结果为南昌霉素 A 发酵研究提供了较为简单、快速、准确的测定方法.
In this paper, biological and chemical methods for the determination of the valence of Nanchangmycin A were studied. Bioassay screened for susceptible strains of Nanchangmycin A, the optimal medium and pH buffer. At 5 to 50 μg/ml Within the range of concentration, the diameter of the inhibitory zone of the susceptible strain was linear with the logarithm of the concentration of Nanchangmycin A. In the chemical measurement, a coloring agent that develops the color of Nanchangmycin A in ethanol was explored, and the most suitable display was found. Color conditions and treatment methods for the sample to be tested. The solution of Nanchangmycin A and the color developing agent showed red color, stable color and strong specificity. The maximum absorption wavelength of the colored solution after the 721 spectrophotometer was 520 millimeters. At micrometers, the absorbance in the range of 5 to 80 μg/ml is proportional to the concentration of Nanchangmycin A, which is in accordance with the Lambert-Beer law. The contents of Nanchangmycin A in the same fermentation broth were determined using the methods of addition of the Nanchangmycin A standard and the standard curve method. The results obtained were very close, indicating that the confidence of this method is 99% and the sample titer The whole process of the assay can be completed within 1 hour. The results of this study provide a simple, rapid and accurate method for the fermentation of Nanchangmycin A.