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目的研究多氯联苯1254对苯并(a)芘致HepG2细胞DNA损伤的影响。方法设11.5、23.0和46.0μmol/L多氯联苯1254剂量组,苯并(a)芘50μmol/L剂量组,10 m l/L二甲基亚砜为溶剂对照。用不同浓度多氯联苯1254预处理HepG2细胞,24 h后染毒,通过单细胞凝胶电泳和高效液相-电化学技术,检测细胞中的DNA链断裂和8-羟基脱氧鸟嘌呤核苷酸(8-OHdG)。结果50μmol/L苯并(a)芘诱导HepG2细胞中DNA O liver尾矩(OTM)值为1.66±0.21,8-OHdG含量为(23.31±6.02)8-OHdG/106dG,溶剂对照组OTM值为0.79±0.15,8-OHdG含量为(12.31±3.24)8-OHdG/106dG,两组比较差异均有统计学意义;11.5、23.0和46.0μmol/L单独处理组OTM值分别为0.88±0.20、1.01±0.15和1.10±0.16,8-OHdG含量分别为(19.57±7.57)、(22.80±9.16)和(31.74±9.25)8-OHdG/106dG,46.0μmol/L组与溶剂对照组比较,8-OHdG含量显著增加,差异有统计学意义;经11.5、23.0和46.0μmol/L的多氯联苯1254预处理,苯并(a)芘诱导的OTM值分别为2.14±0.22、2.43±0.32和2.71±0.31,8-OHdG含量分别为(32.50±3.81)、(49.23±16.66)和(60.36±18.04)8-OHdG/106dG,与苯并(a)芘单独作用组比较均显著增加,差异有统计学意义。结论多氯联苯1254能使苯并(a)芘诱导的HepG2细胞DNA损伤作用显著增强,表明多氯联苯1254对苯并(a)芘的遗传毒性作用具有一定的协同效应。
Objective To study the effect of PCBs 1254 on DNA damage in HepG2 cells induced by benzo (a) pyrene. METHODS: The control group consisted of 125, 25.0 and 46.0 μmol / L PCBs, 50 μmol / L benzo (a) pyrene and 10 mℓ / L dimethyl sulfoxide. HepG2 cells were pretreated with different concentrations of polychlorinated biphenyls (PCBs) 1254 and then exposed to 24 hours after infection. The DNA strand breaks and 8-hydroxydeoxyguanine riboside were detected by single cell gel electrophoresis and high performance liquid chromatography-electrochemical techniques Acid (8-OHdG). Results The OTM value of HepG2 cells induced by 50μmol / L benzo (a) pyrene was 1.66 ± 0.21 and the content of 8-OHdG was (23.31 ± 6.02) 8-OHdG / 106dG. The OTM value of solvent control group was 0.79 ± 0.15, 8-OHdG content was (12.31 ± 3.24) 8-OHdG / 106dG, the difference between the two groups was statistically significant; OTM values of 11.5,23.0 and 46.0μmol / L alone treatment group were 0.88 ± 0.20,1.01 ± 0.15 and 1.10 ± 0.16, 8-OHdG were (19.57 ± 7.57), (22.80 ± 9.16) and (31.74 ± 9.25) 8-OHdG / 106dG respectively. Compared with the solvent control group, (P <0.05). The OTM values induced by benzo (a) pyrene were 2.14 ± 0.22, 2.43 ± 0.32 and 2.71 ± respectively after pretreatment with PCBs at 11.5, 23.0 and 46.0 μmol / L 0.31 and 8-OHdG were (32.50 ± 3.81), (49.23 ± 16.66) and (60.36 ± 18.04) 8-OHdG / 106dG, respectively, which were significantly higher than that of benzo (a) pyrene group significance. Conclusion Polychlorinated biphenyls (PCBs) 1254 can significantly enhance the DNA damage induced by benzo [a] pyrene in HepG2 cells, indicating the synergistic effect of PCBs 1254 on the genotoxicity of benzo [a] pyrene.