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为了解婴儿先天性巨结肠（ＨＤ）与巨细胞病毒（ＣＭＶ）感染的关系，对１９例２～１８个月的ＨＤ患儿（经病理证实）的血清、尿和组织块用病毒分离和ＤＮＡ探针杂交方法进行ＣＭＶ检测。结果：１９例ＨＤ患儿分离ＣＭＶ的１９份尿标本中２份污染，在１７份尿标本中７份阳性，阳性率为４１．２％；正常儿尿标本阳性率为１４．４％（Ｐ＜０．０５）。１９例ＨＤ血清中，ＣＭＶＩｇＭ抗体２例阳性，阳性率为１０．５％；正常儿阳性率为８．０％（Ｐ＞０．０５）。１９例ＨＤ痉挛段组织块中无一例ＣＭＶ阳性，但６例ＨＤ痉挛段组织块ＣＭＶ－ＤＮＡ探针杂交全部阳性，其中２例强阳性，阳性率为１００％。说明婴儿ＨＤ与ＣＭＶ感染关系密切，ＣＭＶ感染可能是包括ＨＤ在内的一些先天性畸形的重要致畸因素。
To understand the relationship between Hirschsprung’s disease and cytomegalovirus (CMV) infection, serum, urine and tissue masses from 19 children aged 2 to 18 months (pathologically confirmed) were isolated using DNA and DNA Probe hybridization method CMV test. Results: Two of 19 urine samples from 19 cases of HD were contaminated by CMV, 7 were positive in 17 urine samples, the positive rate was 41.2%. The positive rate of normal urine samples was 14.4% (P <0.05). In 19 cases of HD serum, CMVIgM antibody was positive in 2 cases, the positive rate was 10.5%. The positive rate of normal children was 8.0% (P> 0.05). None of the 19 cases of HD spasticity had CMV positive, but 6 cases of HD spasticity showed positive CMV-DNA hybridization, of which 2 were strongly positive and the positive rate was 100%. This shows that infant HD is closely related to CMV infection, and CMV infection may be an important teratogenic factor of some congenital malformations including HD.