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目的开展手足口病病原学检测,了解乌鲁本齐市2010年手足口病病原特点。方法用逆转录聚合酶链反应(RT-PCR)法,对208例手足口病患者的咽拭样本105份、粪便样本104份、肛拭样本27份共236份进行肠道病毒71型(EV71)和柯萨奇病毒A组(CA16)的检测。结果实验室检测的208例样本中,EV71检出104例,CA16检出66例,其他肠道病毒检出14例。病原监测发现2010年以EV71和CA16病原流行为主,各月监测的病例均以EV71型较多,各区县均监测到EV71病原。结论 RT-PCE方法用于手足口病EV71和CA16病原的检测,可以快速、准确地扩增咽拭、粪便及肛拭中的病毒,是一种快速、特异的检测方法。2010年乌鲁木齐市手足口病疫情自5月以来有明显增高趋势,呈现散发为主、局部聚集性发病的特点,病原主要是EV71和CA16型病毒。
Objective To carry out pathogen detection of hand-foot-mouth disease and understand the characteristics of hand-foot-mouth disease in Urumqi in 2010. Methods Totally 236 pharyngeal swab samples from 105 cases of 208 hand-foot-mouth disease patients, 104 fecal samples and 27 samples of anal swabs were detected by RT-PCR. ) And Coxsackie virus A (CA16). Results Of the 208 samples tested in the laboratory, 104 were found in EV71, 66 in CA16 and 14 in other enteroviruses. Pathogen surveillance found that in 2010 the prevalence of EV71 and CA16 pathogens, the monthly monitoring of EV71-type cases are more, all districts and counties were detected EV71 pathogen. Conclusion The RT-PCE method can be used to detect EV71 and CA16 pathogens in hand-foot-mouth disease, and can rapidly and accurately amplify the virus in pharyngeal swabs, stools and anal swabs. It is a rapid and specific detection method. In 2010, the epidemic situation of HFMD in Urumqi has been significantly increased since May, showing the characteristics of exuding predominance and local aggregation. The pathogens are mainly EV71 and CA16 viruses.